酸枣仁汤治疗围绝经期失眠症网络药理学机制探讨

Study on the Mechanism of Action of Suanzaoren Decoction in the Treatment of Perimenopausal Insomnia Based on Network Pharmacology

目的:运用网络药理学对酸枣仁汤治疗围绝经期失眠症的机制进行探讨。方法:通过TCMSP数据库获得酸枣仁汤有效成分和靶点,借助GeneCards、disGeNET和TTD数据库获得围绝经期失眠症靶点,利用Venny图得到两者的交集靶点后进行中药-活性成分-有效靶点网络和PPI网络构建,GO和KEGG富集分析,采用Discovery Studio 2016进行分子对接验证。结果:经过筛选得到酸枣仁有效成分116种,237个靶点数,203个疾病靶点,38个交集靶点,活性成分主要为槲皮素、山奈酚、芒柄花素、柚皮素、豆甾醇,核心靶点为IL-6、TNF、IL-1β、ESR1、PPARG。涉及BP265个条目、CC19个条目、MF46个条目,KEGG生物通路80条。分子对接结果验证了活性成分和核心靶点之间能自发结合,且相互作用较强。结论:通过网络药理学筛选出活性成分和核心靶点,为开展基础研究深入探讨酸枣仁汤治疗围绝经期失眠症提供参考。

Objective:To investigate the mechanism of Suanzaoren decoction in treating perimenopausal insomnia by network pharmacology.Method:Effective components and targets of Suanzaoren decoction were obtained by TCMSP database,perimenopausal insomnia targets were obtained by GeneCards,disGeNET and TTD databases,and the intersection targets of the two were obtained by Venny diagram.After the construction of TCM-active ingredients-effective target network and PPI network,GO and KEGG enrichment analysis were carried out.Discovery Studio 2016 was used for molecular docking verification.Result:After screening,116 kinds of active components of jujube kernel were obtained,237 target numbers,203 disease targets,38 intersection targets.The main active ingredients are quercetin,kaempferol,formononetin,naringenin,stigmasterol.The core targets were IL-6,TNF,IL-1β,ESR1 and PPARG.It involved BP265 items,CC19 items,MF46 items,and 80 KEGG biological pathways.The results of molecular docking verified that the active ingredient and the core target could spontaneously bind,and the interaction was strong.Conclusion:The active ingredients and core targets were screened through network pharmacology to provide reference for the basic research of Suanzaoren decoction in the treatment of perimenopausal insomnia.