酸性有机溶剂预处理改善木质纤维素酶解及发酵性能

Co-solvent combined with organic acid pretreatment to improve the enzymatic hydrolysis and fermentation performance of lignocellulose

试验通过比较不同助溶剂/水体系中、不同有机酸催化作用下,玉米秸秆组分分离效果以及纤维素回收情况,建立酸性有机溶剂预处理法,并对预处理后得到的富含纤维素的固体进行了酶水解和同步糖化发酵,以评价该预处理方法对后期酶解和发酵的影响。结果显示,在丙酮/水溶剂(50wt%)体系中,以1wt%草酸作为催化剂,150℃下预处理30 min,可脱除91.5%的半纤维素和77.0%的木质素,纤维素的保留率达到88.7%。回收得到的玉米秸秆经酶水解,其纤维素转化率可达84.5%,相比未处理的玉米秸秆提高了62.4%。研究表明,以有机酸为催化剂的助溶剂/水预处理反应体系有效改善了玉米秸秆酶解及发酵性能,为木质纤维素原料的高值化利用提供了一定参考。

The experiment aimed to establish an acidic organic solvent pretreatment method by comparing the separation effects of corn straw components and the recovery of cellulose in different solvent/water systems under the catalytic action of various organic acids.The pretreated cellulose-rich solid obtained was subjected to enzymatic hydrolysis and simultaneous saccharification and fermentation to evaluate the impact of this pretreatment method on subsequent enzymatic hydrolysis and fermentation.The results showed that in an acetone/water solvent system(50wt%)with oxalic acid as a catalyst at 1wt%,a pretreatment at 150℃for 30 minutes could remove 91.5%of hemicellulose and 77.0%of lignin,with a cellulose retention rate of 88.7%.The recovered corn straw,after enzymatic hydrolysis,achieved a cellulose conversion rate of 84.5%,which is a 62.4%increase compared to untreated corn straw.The study indicates that the solvent/water pretreatment reaction system with organic acid as a catalyst effectively improved the enzymatic hydrolysis and fermentation performance of corn straw,providing a reference for the high-value utilization of lignocellulosic materials.