安岳红小麦重组自交系产量相关性状的QTL分析

Quantitative Trait Locus Analysis on Yield Related Traits Using Recombinant Inbred Lines Derived from a Bread Wheat Variety Anyuehong

为解析小麦产量相关性状的遗传效应,以四川本地高抗病、多小穗、高粒重品种安岳红小麦,与台长29杂交后构建114份重组自交系,利用小麦55K SNP芯片进行全基因组扫描,对2年3重复下的小穗数、穗长、有效分蘖、小穗粒数、穗粒数、穗粒重和千粒重7个产量相关性状进行表型鉴定及最佳线性无偏预测(BLUP),对表型数据进行QTL定位。共检测到32个控制小麦产量相关性状QTL,可解释表型变异率为4.67%~24.43%。其中,25个QTL可解释表型变异率超过10%,分布于1A、2A、2B、2D、3A、3D、4A、4D、5A、5D、6B、6D和7D染色体上。位于4D染色体上控制穗长的QSLAYH.Sicau-4D.1,在2个实验点中均能稳定表达。同时发现,1A、3A和4D染色体上分别携带有1个具有一因多效QTL。这说明小麦的产量性状遗传变异丰富,定位的优良等位基因具有增加小穗数、小穗粒数、穗粒数、穗粒重和千粒重的作用。

To analyze the genetic effects of yield-related traits in wheat,a total of 114 recombinant inbred lines were derived from a cross between a wheat landrace Anyuehong with high disease resistant,multi-spikelets,and high grain weight and Taichang 29.The wheat 55K SNP array was used for whole genome screening to identify phenotype and perform optimal linear unbiased prediction(BLUP)on seven yield-related traits,including spikelet number,spike length,effective tillering,spikelet grain number,grain number per spike,grain weight per spike,and thousand-grain weight,at three locations for two years.QTL mapping was performed on the phenotype data.A total of 32 QTLs related to wheat yield were detected,with the rate from 4.67%to 24.43%explaining the phenotype variations.Among them,25 QTLs can explain phenotypic variation rates over 10%,distributed on chromosomes 1A,2A,2B,2D,3A,3D,4A,4D,5A,5D,6B,6D,and 7D.Among them,QSLAYH.Sicau-4D.1,located on the 4D chromosome,controls spike length,which was observed at both experimental locations.Meanwhile,it was found that chromosomes 1A,3A,and 4D individually carry one pleiotropism QTL.Wheat yield traits had abundant genetic variations,and the identified prior alleles have the functions of increasing spikelet number,grain number per spikelet,grain number per spike,grain weight per spike,and thousand-grain weight.