重庆武隆发现贵州臭蛙

Odorrana kweichowensis Found in Wulong,Chongqing,China

2022年8月2日在重庆市武隆区(29°32′29″N,107°53′15″E,海拔697 m)采集到5号两栖动物个体,经形态特征比较,与贵州臭蛙(Odorrana kweichowensis)相似;基于线粒体ND2基因构建臭蛙属部分物种系统发育树,此次采集的臭蛙标本与贵州臭蛙聚为一支,且具有高支持率(贝叶斯法/最大似然法:1.00/100);基于Kimura双参数模型估算本次采集的臭蛙标本与贵州金沙贵州臭蛙模式产地标本间的遗传距离为0.03%,远小于臭蛙属物种间的遗传距离(6.56%~24.38%)。综合形态特征和分子系统发育比较,确定此次采集到的臭蛙标本为无尾目(Anura)蛙科(Ranidae)臭蛙属的贵州臭蛙,系重庆市两栖动物分布新记录种。

During the amphibian survey in Wulong District,Chongqing City,five specimens were collected on 2 August,2022(29°32′29″N,107°53′15″E,altitude 697 m)(Fig.1).Compared the specimens with other species of the genus Odorrana by morphological and molecular evidences(Table 1),the specimens were identified as Odorrana kweichowensis,which is the new record of Chongqing,China.Morphologically,the specimens collected from Wulong appeared to be similar with O.kweichowensis(Fig.2),the snout vent length of the males and females collected from Wulong were longer than that of the topotype(Table 2);molecularly,we constructed phylogenetic trees using Bayesian inference(BI)and maximum likelihood(ML)methods based on ND2 gene and calculated the genetic distances between species of the genus Odorrana using Kimura 2-parameter model in MEGA v6.0.The BI and ML trees based on ND2 gene presented almost consistent topology,showing that the five specimens from Chongqing were clustered with O.kweichowensis with high support(1.00/100)(Fig.3).The genetic distance between the specimens from Chongqing and O.kweichowensis from type locality was 0.03%,much lower than that between Odorrana species,ranging from 5.56%to 24.38%(Table 3).The new record of O.kweichowensis in Chongqing would promote taxonomic and phylogeographic studies on this species.