线粒体转录延伸因子在人脑胶质瘤组织中的表达及预后意义

Expression and prognostic significance of mitochondrial transcription elongation factor in human brain glioma tissues

目的:研究TEFM基因在人脑胶质瘤组织中的表达情况,并探讨其与脑胶质瘤患者临床病理特征及预后的相关性。方法:采集我校附属医院2019年01月至2021年06月经手术切除后病理确认的68例脑胶质瘤组织及35例非肿瘤脑组织。采用qRT-PCR、Western blot法和免疫组化SP法检测脑胶质瘤组织和非肿瘤脑组织中TEFM mRNA和蛋白的表达水平。分析TEFM基因mRNA表达量与脑胶质瘤患者临床病理参数的相关性。根据随访资料采用Kaplan-Meier生存曲线分析TEFM mRNA表达量和患者预后的相关性。采用COX回归模型分析患者预后的独立风险因素。结果:免疫组织化学结果显示,TEFM蛋白主要表达于细胞质,且在不同级别脑胶质瘤组织中的阳性表达率(91.18%)明显高于非肿瘤脑组织中的阳性表达率(37.14%),组间差异极显著(P<0.01)。Western blot和qRT-PCR结果显示,TEFM蛋白和mRNA在脑胶质瘤组织中均显著增高(P<0.01)。脑胶质瘤患者TEFM基因mRNA表达量与患者年龄、病理分级、病理类型、偏侧性及幕上位置均显著相关(P<0.05);Kaplan-Meier生存分析显示,TEFM mRNA表达水平显著影响脑胶质瘤患者的总生存期(overall survival,OS)和无病生存期(disease-free survival,DFS),TEFM高表达的患者OS和DFS显著缩短(Log-rank P<0.05);根据COX多因素回归模型分析表明,年龄、病理分级、病理类型和异柠檬酸脱氢酶(IDH1)突变情况是对脑胶质瘤患者预后造成影响的独立风险因素(P<0.05)。结论:与非肿瘤脑组织相比,TEFM蛋白和mRNA在脑胶质瘤组织中表达均明显增高,且TEFM高表达的患者OS和DFS明显缩短。

Objective:To investigate the expression of human mitochondrial transcription elongation factor(TEFM)in glioma tissues and its relationship with clinicopathological features and prognosis.Methods:From January 2019 to June 2021,68 cases of glioma tissues and 35 cases of non-tumorous brain tissues surgical resected and confirmed by pathology were collected.Immunohistochemistry and Western blot was used to determine the expressions of TEFM in glioma tissues and non-tumorous brain tissues.Real-time fluorescence quantitative RT-PCR(qRT-PCR)was used to detect the mRNA level of TEFM in the above tissues.The expression and distribution of TEFM mRNA in different clinicopathological features of glioma(age,sex,pathological type,pathological grade,history of headache,laterality,and supratentorial location)were analyzed.The relationship between TEFM mRNA level and prognosis was analyzed according to follow-up data.Results:Immunohistochemical analysis revealed that TEFM protein is predominantly localized in the cytoplasm.The positive expression rate of TEFM in various grades of brain glioma tissues(91.18%)significantly surpassed that in non-tumor brain tissues(37.14%),showing a highly significant intergroup difference(P<0.01).Western blot and qRT-PCR results showed that both TEFM protein and mRNA were significantly increased in glioma tissue(P<0.01).The expression of TEFM gene in glioma patients was significantly correlated with age,pathological grade,pathological type,laterality and supratentorial location(P<0.05).Kaplan-Meier survival analysis showed that the expression of TEFM gene significantly affected the overall survival(OS)and disease-free survival(DFS)of glioma patients,while the OS and DFS of patients with high expression of TEFM were significantly shortened(Log-rank P<0.05).Using the COX regression model to analyze the independent risk factors for the prognosis of patients with gliomas,age,pathological grade,pathological type and IDH1 mutation status are independent risk factors that affect the prognosis of glioma patients(P<0.05).Conclusion:TEFM is significantly up-regulated in human brain glioma tissues compared to non-tumorous brain tissues.Patients with high TEFM expression have significantly shorter OS and DFS.