布渣叶不同极性溶剂萃取物抗菌及抗氧化活性研究

Research on antibacterial and antioxidant activity of different polar solvent extracts of Microctis folium

[目的]进一步开发布渣叶的抗氧化活性功能。[方法]采用溶剂萃取法得到布渣叶石油醚萃取物、二氯甲烷萃取物、乙酸乙酯萃取物和水萃取物,测定其对院内感染常见细菌的抗菌活性,并通过测定不同极性萃取物对DPPH自由基、羟自由基及ABTS自由基的清除能力来评价其抗氧化活性。[结果]各萃取物对金黄色葡萄球菌和鲍曼不动杆菌有明显抗菌作用,其中乙酸乙酯萃取物和水萃取物活性最佳,但对肺炎克雷伯菌、大肠杆菌、铜绿假单胞菌没有抑菌作用。水萃取物对DPPH自由基的清除效果最好,其IC50值为(0.0932±0.0150)mg/mL;乙酸乙酯萃取物清除羟自由基和ABTS自由基的效果最好,清除羟自由基的IC50值为(0.4517±0.0510)mg/mL。[结论]布渣叶不同溶剂萃取物尤其是乙酸乙酯萃取物和水萃取物具有作为天然抗菌剂和天然抗氧化剂研究与开发的潜力。

[Objective]This study aimed to further develop the antioxidant activity function of Microctis folium leaves.[Methods]M.folium leaves extracts of petroleum ether,dichloromethane,ethyl acetate and water were obtained by solvent extraction to determine their antimicrobial activities against common bacteria in nosocomial infections,and the antioxidant activities were evaluated by determining the scavenging capacities of the extracts of different polarities against DPPH radicals,hydroxyl radicals and ABTS radicals.[Results]The extracts showed significant antibacterial effects against Staphylococcus aureus and Acinetobacter baumannii,with the best activity of ethyl acetate extract and water extract,but no inhibitory effect against Klebsiella pneumoniae,Escherichia coli and Pseudomonas aeruginosa.The aqueous extract had the best scavenging effect on DPPH radicals with an IC50 value of(0.0932±0.0150)mg/mL,while the ethyl acetate extract had the best scavenging effect on hydroxyl and ABTS radicals,with an IC50 value of(0.4517±0.0510)mg/mL for scavenging hydroxyl radicals.[Conclusion]The different solvent extracts of M.folium,especially ethyl acetate extract and water extract,have the potential to be researched and developed as natural antimicrobial agents and natural antioxidants.