OLR1在卵巢癌中的表达及其与免疫细胞浸润、预后的相关性分析

The expression of OLR1 and its correlation with immune cell invasion and prognosis in ovarian cancer

目的应用生物信息学工具分析氧化低密度脂蛋白受体1(oxidized low density lipoprotein receptor 1,OLR1)基因在卵巢癌中的表达特征,并探讨其与免疫细胞浸润、患者预后的关联。方法采用GEPIA2、GeneMANIA、STRING、cBioPortal、LinkedOmics、TIMER 2.0、TISIDB数据库和Kaplan-Meier生存曲线探讨OLR1基因在卵巢癌组织和正常卵巢组织中的表达差异及其与预后的相关性;分析OLR1的共表达基因和蛋白互作网络,分析OLR1基因在卵巢癌中的基因突变情况;分析OLR1表达与肿瘤免疫细胞浸润、免疫调节因子间的相关性。结果卵巢癌组织OLR1呈显著高表达,高表达的OLR1显著缩短卵巢癌患者的总生存期(overall survival,OS)、无进展生存期(progression-free survival,PFS)和疾病进展后生存期(post-progression survival,PPS)(P<0.05);OLR1表达与CD8^(+)T细胞、树突状细胞、巨噬细胞、中性粒细胞浸润呈正相关,与大多数免疫抑制剂、免疫刺激剂和主要组织相容性抗体相关分子呈正相关。结论OLR1基因可作为卵巢癌诊断、预后和免疫细胞浸润水平的标志物。

Objective To analyze oxidized low density lipoprotein receptor 1(OLR1)expression characteristics and its correlation with immune cell infiltration and prognostic outcomes in ovarian cancer by bioinformatics methods.Methods GEPIA2,GeneMANIA,STRING,cBioPortal,LinkedOmics,TIMER 2.0 and TISIDB databases and Kaplan-Meier survivorship curve were used to examine the differential expression of the OLR1 gene between ovarian cancer tissues and normal ovarian tissues and its association with prognosis,and they were used to analyze co-expressed genes and protein-interaction networks of OLR1,the gene mutation status in ovarian cancer,and the correlation between OLR1 expression and tumor immune cell infiltration and immune modulator expression levels.Results The expression of OLR1 was significantly higher in ovarian cancer tissues,and the high expression of OLR1 significantly shortened overall survival(OS),progression-free survival(PFS)and post-progression survival(PPS)(P<0.05).OLR1 expression was positively correlated with infiltration of CD8^(+)T cells,dendritic cells,macrophages,neutrophils,and most immunosuppressants,immunostimulants,and major histocompatibility complex associated molecules.Conclusion The OLR1 gene can serve as a biomarker for the diagnosis,prognosis,and evaluation of immune cell infiltration levels in ovarian cancer.