RAP1B对类风湿关节炎成纤维样滑膜细胞生物学行为的影响研究

The effects of RAP1B on fibroblast-like synoviocytes in rheumatoid arthritis

目的探讨RAP1B对体外培养的类风湿关节炎(rheumatoid arthritis,RA)成纤维样滑膜细胞(fibroblast-1ike synoviocyte,FLS)增殖、迁移以及炎症因子分泌能力的影响。方法通过构建RAP1B过表达及干扰表达的慢病毒载体,转染至人RA成纤维样滑膜细胞(MH7A),分别采用CCK8法、划痕实验、Transwell-迁移实验及ELISA法,对MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力进行检测。结果RAP1B表达上调后,MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力均明显增强(均P<0.01);RAP1B表达下调后,MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力均明显减弱(均P<0.05)。结论RAP1B具有促进RA-FLS增殖、迁移及炎症因子分泌的能力。

Objective To investigate the impact of RAS-related protein 1B(RAP1B)on the proliferation,migration,and inflammatory factor secretion of fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA).Methods We constructed lentiviral vectors for both overexpression and interference expression of RAP1B,which were then stably transfected into human RA-FLS(MH7A).The proliferation,migration,and secretion of inflammatory factors(IL-1β,IL-6,TNF-α,IL-8)of MH7A cells were detected by the CCK8 method,scratch test,Transwell-migration test,and ELISA,respectively.Results Up-regulation of RAP1B enhanced the proliferation,migration,and inflammatory factor secretion of MH7A cells,while down-regulation of RAP1B had the opposite effect.Conclusion RAP1B has the capacity to promote the proliferation,migration,and secretion of inflammatory factors(IL-1β,IL-6,TNF-α,and IL-8)in RA-FLS.