蛋氨酸锌对断奶仔猪生长性能、肠道微生物及免疫力的影响

Effects of zinc methionine on growth performance,intestinal microorganisms,and immunity of weaned piglets

试验旨在研究不同水平的蛋氨酸锌对断奶仔猪生长性能、肠道微生物及机体免疫力的影响。选择160头21日龄的断奶仔猪,随机分为4组,每组4个重复,每个重复10头仔猪。各组分别饲喂添加0(对照组)、50(试验A组)、100(试验B组)和200 mg/kg(试验C组)蛋氨酸锌的饲粮。预试期1 w,正式试验期4 w。结果表明:试验B组仔猪末重高于对照组(P<0.05),料重比低于对照组(P<0.05),试验B组仔猪平均日增重高于试验A组和对照组(P<0.05),所有蛋氨酸锌组仔猪的腹泻率均低于对照组(P<0.05)。与对照组相比,试验B组仔猪肠道软壁菌门、拟杆菌门相对丰度升高(P<0.05),试验A组和试验B组肠道厚壁菌门相对丰度升高(P<0.05)。与对照组比较,试验B组和试验C组仔猪肠道梭菌属相对丰度减少(P<0.05);所有蛋氨酸锌组瘤胃球菌属相对丰度显著降低(P<0.05),乳杆菌属相对丰度显著增加(P<0.05)。与对照组比较,试验B组仔猪血清免疫球蛋白G含量增加(P<0.05),白细胞介素-6含量降低(P<0.05),肿瘤坏死因子-α含量降低(P<0.05);所有蛋氨酸锌组仔猪血清白细胞介素-10含量增加(P<0.05);试验B组仔猪血清中内毒素和D-乳酸含量均降低(P<0.05),所有蛋氨酸锌组仔猪血清二胺氧化酶活性降低(P<0.05)。研究表明,蛋氨酸锌可以提高仔猪的生长性能、免疫力,改善肠道健康,且蛋氨酸锌的适宜添加水平为100 mg/kg。

The experiment aimed to investigate the effects of different levels of zinc methionine on the growth performance,intestinal microbiota,and immunity of weaned piglets.A total of 160 weaned piglets at 21 days of age were randomly divided into four groups,with four replicates per group and 10 piglets per replicate.The groups were fed diets supplemented with 0(control group),50(experiment A group),100(experiment B group),and 200 mg/kg(experiment C group)of zinc methionine.The preliminary trial period was one week,and the formal experimental period was four weeks.The results showed that the final weight of piglets in experiment B group was higher than that of the control group(P<0.05),and the feed-to-gain ratio was lower than that of the control group(P<0.05).The average daily weight gain of piglets in experiment B group was higher than that of experiment A group and control group(P<0.05),and the diarrhea rate of all zinc methionine groups was lower than that of the control group(P<0.05).Compared with the control group,the relative abundance of Tenericutes and Bacteroidetes in the gut of piglets in experiment B group increased(P<0.05),and the relative abundance of Firmicutes in the gut of experiment A group and experiment B group increased(P<0.05).Compared with the control group,the relative abundance of Clostridium in the gut of piglets in experiment B group and experiment C group decreased(P<0.05);the relative abundance of Ruminococcus in the gut of all zinc methionine groups significantly decreased(P<0.05),and the relative abundance of Lactobacillus significantly increased(P<0.05).Compared with the control group,the level of immunoglobulin G in the serum of piglets in experiment B group increased(P<0.05),the content of interleukin-6 decreased(P<0.05),and the content of tumor necrosis factor-αdecreased(P<0.05),the content of interleukin-10 in the serum of all zinc methionine groups increased(P<0.05),the concentration of endotoxin and D-lactic acid in the serum of piglets in experiment B group decreased(P<0.05),and the activity of diamine oxidase in the serum of all zinc methionine groups decreased(P<0.05).The study indicates that zinc methionine can improve the growth performance and immunity of piglets,and improve intestinal health,with an appropriate addition level of 100 mg/kg.