亚麻籽木脂素对初产大鼠乳腺发育及泌乳性能的影响

Effects of flaxseed lignans on mammary gland development and lactation performance in primiparous rats

试验旨在研究亚麻籽木脂素(SDG)对初产大鼠乳腺发育、泌乳性能及泌乳相关激素水平的影响。选用初产大鼠80只,随机分为4组(20只/组)。对照组大鼠饲喂正常日粮,试验Ⅰ组、Ⅱ组、Ⅲ组除了饲喂正常日粮外,分别灌服125 mg/(L·kg)SDG、5 mg/(L·kg)甲磺酸溴隐亭片(Br)、125 mg/(L·kg)SDG+5 mg/(L·kg)Br,SDG妊娠全期灌服,Br从妊娠第14 d起至分娩给药,分娩当日停药,每窝留8只仔鼠用于测定产奶性能。试验期15 d。结果显示,试验Ⅰ组、Ⅲ组13、15 d的产奶量均明显高于试验Ⅱ组(P<0.01或P<0.05)。试验Ⅰ组大鼠的乳腺重、乳腺DNA和RNA含量以及乳腺RNA/DNA的比值显著高于对照组(P<0.05),极显著高于试验Ⅱ组(P<0.01)。试验Ⅰ组和Ⅲ组大鼠的乳腺腺泡直径显著高于对照组和试验Ⅱ组(P<0.05),导管直径极显著高于对照组和试验Ⅱ组(P<0.01)。试验Ⅰ组大鼠的性腺轴激素水平均明显高于其他组,其中雌二醇(E2)、催乳素(PRL)含量显著高于试验Ⅱ组(P<0.05),孕酮(P)、促卵泡激素(FSH)、生长激素(GH)含量极显著高于试验Ⅱ组(P<0.01)。研究表明,灌服SDG可以明显改善大鼠乳腺功能,促进乳腺腺泡及导管的发育,提高大鼠产奶量,提高泌乳相关激素水平;不同处理组对初产大鼠乳腺作用情况为试验Ⅰ组>试验Ⅲ组>对照组>试验Ⅱ组。

The experiment aimed to investigate the effects of flaxseed lignans(SDG)on mammary gland development,lactation performance,and lactation-related hormone levels in primiparous rats.A total of 80 primiparous rats were randomly divided into four groups(20 rats per group).The control group was fed a normal diet,while the test groups Ⅰ,Ⅱ,and Ⅲ were fed a normal diet plus orally administered 125 mg/(L·kg)SDG,5 mg/(L·kg)Br,and 125 mg/(L·kg)SDG+5 mg/(L·kg)Br,respectively.SDG was administered throughout pregnancy,and Br was given from the 14th day of gestation until delivery.Medication was stopped on the day of delivery,and eight pups from each litter were retained for milk production measurement.The experimental period lasted for 15 days.The results showed that the milk production on days 13 and 15 of groups Ⅰ and Ⅲ was significantly higher than that of group Ⅱ(P<0.01 or P<0.05).The mammary gland weight,mammary DNA and RNA content,and the RNA/DNA ratio in test group Ⅰ were significantly higher than those in the control group(P<0.05),and extremely higher than those in test group Ⅱ(P<0.01).The acini diameter in the mammary glands of rats in test groups Ⅰ and Ⅲ was significantly higher than that in the control group and test group Ⅱ(P<0.05),and the duct diameter was extremely higher than that in the control group and test group Ⅱ(P<0.01).The levels of reproductive axis hormones in group Ⅰ were significantly higher than those in the other groups,with estradiol(E2)and prolactin(PRL)were significantly higher than those in test group Ⅱ(P<0.05),and progesterone(P),follicle-stimulating hormone(FSH),and growth hormone(GH)were extremely higher than those in test group Ⅱ(P<0.01).The study indicates that the administration of SDG can improve rat mammary function,promote the development of mammary acini and ducts,increase milk production,and elevate lactation-related hormone levels.The effects of different treatment groups on the mammary glands of primiparous rats were ranked as follows:Test group Ⅰ>test group Ⅲ>test control group>test group Ⅱ.