植物乳杆菌19595-02的抗霉菌特性

Anti-Mould Activity of Lactobacillus plantarum 19595-02

为将乳酸菌应用到食品和饲料等生物保存剂中,该研究探讨了乳酸菌的抗霉菌活性。以琼脂培养基覆盖法检测筛选出了抗霉菌活性较高而广谱的酸马奶中分离植物乳杆菌19595-02菌株,再用琼脂扩散法对该菌株的抗霉菌活性物质特性探索。结果表明,在培养30℃、48~60 h时活性达到顶峰,80~100℃热处理和pH3.5~4.0范围内表现出稳定性。然而,用胃蛋白酶处理时,残留抗霉菌活性为67%;用胰蛋白酶处理时,失去了抗霉菌活性。因此,推断19595-02菌株产生的抗霉菌物质为蛋白性质的化合物,需进一步研究探讨。

In order to apply lactic acid bacteria to food and feed,the antifungal activity of lactic acid bacteria was studied.Lactobacillus plantarum 19595-02 with high antifungal activity and broad spectrum was screened from koumiss by agar medium covering method.Agar diffusion method was used to explore the characteristics of antifungal active substances.The results showed that the antifungal activity of the strain reached the peak at 30℃and 48–60 h,and it was stable at 80–100℃and pH 3.5–4.0.However,the residual antifungal activity was 67%when treated with pepsin,and lost the antifungal activity when treated with trypsin.Therefore,it is concluded that the antifungal substances produced by 19595-02 strain are protein compounds,which need further study.