糖尿病足感染病原菌及血清IL-17、IL-12p70、IL-8、CRP与感染进展的关系

Pathogenic bacteria in patients with diabetic foot infection and relationship between serum IL-17,IL-12p70,IL-8 and CRP levels and infection progression

目的分析糖尿病足(DF)感染患者病原菌特点及血清白细胞介素(IL)-17、IL-12p70、IL-8、C-反应蛋白(CRP)与感染进展的关系。方法回顾性分析2020年1月-2023年10月安徽省阜阳市肿瘤医院收治的68例DF感染患者(感染组)与68例DF未感染患者(未感染组)的临床资料,检测感染组病原菌,记录两组血清IL-17、IL-12p70、IL-8、CRP水平,评估感染患者感染程度与血清IL-17、IL-12p70、IL-8、CRP水平的相关性。感染组均行规范化综合治疗,观察感染组感染期及感染控制期血清IL-17、IL-12p70、IL-8、CRP水平变化。结果68例DF感染患者共检出病原菌108株,其中革兰阴性菌53株占49.07%,革兰阳性菌48株占44.44%,真菌7株占6.48%;感染组血清IL-17、IL-12p70、IL-8和CRP分别为(22.60±4.22)pg/ml、(4.08±0.83)pg/ml、(332.11±35.19)pg/ml和(16.02±3.45)mg/L均高于未感染组(P<0.05);Spearman相关性分析显示,DF感染程度与血清IL-17、IL-12p70、IL-8、CRP水平呈正相关(r=0.513、0.722、0.896、0.763,均P<0.001);感染组感染控制期血清IL-17、IL-12p70、IL-8和CRP分别为(18.97±3.04)pg/ml、(3.05±0.62)pg/ml、(250.45±33.26)pg/ml和(11.87±2.35)mg/L低于感染期(P<0.05)。结论DF感染患者病原菌以革兰阴性菌居多,血清IL-17、IL-12p70、IL-8、CRP可能参与DF感染的疾病进展及转归。

OBJECTIVE To analyze the characteristics of pathogenic bacteria in patients with diabetic foot(DF)infection and the relationship between serum interleukin(IL)-17,IL-12p70,IL-8 and C-reactive protein(CRP)levels and infection progression.METHODS The clinical data of 68 patients with DF infection(infection group)and 68 patients without DF infection(non-infection group)admitted to Fuyang Cancer Hospital of Anhui Province from Jan.2020 to Oct.2023 were retrospectively analyzed,the pathogenic bacteria in infection group were detected,the levels of serum IL-17,IL-12p70,IL-8 and CRP in the two groups were recorded,the correlation between degree of infection and levels of serum IL-17,IL-12p70,IL-8 and CRP in infected patients was evaluated.The infection group was treated with standardized comprehensive treatment.The changes of serum IL-17,IL-12p70,IL-8 and CRP levels in infection group during infection period and infection control period were observed.RESULTS A total of 108 strains of pathogenic bacteria were detected in 68 patients with DF infection,of which 53 strains of gram negative accounted for 49.07%,48 strains of gram positive accounted for 44.44%,and 7 strains of fungi accounted for 6.48%;The serum levels of IL-17,IL-12p70,IL-8,and CRP in the infection group were(22.60±4.22)pg/ml,(4.08±0.83)pg/ml,(332.11±35.19)pg/ml and(16.02±3.45)mg/L,respectively,higherthan those in the non-infection group(P<0.05).Spearman rank correlation analysis showed that the degree of DF infection was positively correlated with serum IL-17,IL-12p70,IL-8,and CRP levels(r=0.513,0.722,0.896,0.763,all P<0.001);The serum levels of IL-17,IL-12p70,IL-8,and CRP in the infection group during the infectioncontrolperiodwere(18.97±3.04)pg/ml,(3.05±0.62)pg/ml,(250.45±33.26)pg/ml,and(11.87±2.35)mg/L,respectively,lower than those of the infection period(P<O.05).CONCLUSIONS Gram-negative bacteria were the most common pathogenic bacteria in patients with DF infection.Serum IL-17,IL-12p70,IL-8 and CRP might be involved in the disease progression and outcomes of DF infection.