重视葡聚糖硫酸钠诱导结肠炎模型的病理学评价

Emphasis is Placed on Pathological Evaluation of Dextran Sulfate Sodium-Induced Colitis Model

化学诱导小鼠结肠炎模型是人类炎症性肠病最常用的动物模型,其中葡聚糖硫酸钠诱导的结肠炎模型应用最广。在葡聚糖硫酸钠诱导模型制备中,不同浓度的葡聚糖硫酸钠、不同品系的小鼠,在不同小鼠的肠段所呈现的病变严重程度不一样。因此,取某一肠段的样本进行病理学评价是不合理的,应该将整个大肠制成“瑞士卷”,分上段、中段和下段结肠全面评价病理改变。肛管病变严重,应该单独评价。全面正确评价肠道病变,才能正确评价药物的治疗效果。

The chemically induced mouse colitis model is the most commonly used animal model for human inflammatory bowel disease,among which dextran sulfate sodium induced colitis model is the most widely used。In the preparation of a dextran sulfate induced model,different concentrations of dextran sulfate and different strains of mice showed varying degrees of lesion severity in the intestinal segments of different mice.Therefore,it is unreasonable to take a sample of a certain segment of the colon for pathological evaluation.The entire colon should be made into a“Swiss roll”and the pathological changes should be comprehensively evaluated by dividing it into upper,middle and lower segments of the colon.The anal canal lesion is severe,it should be evaluated separately.Only by comprehensively and correctly evaluating the intestinal lesion can the therapeutic effect of drugs be correctly evaluated.