Myo1E在胰腺导管腺癌组织中的表达及临床意义

Expression and clinical significance of Myo1E in pancreatic ductal adenocarcinoma tissues

目的:分析肌球蛋白1E(Myo1E)在胰腺导管腺癌(PDAC)组织中的表达及临床意义。方法:用在线癌症基因组图谱数据库中PDAC的二代测序数据,分析Myo1E mRNA在PDAC组织中的表达水平,与患者预后和免疫检查点的关系;用PDAC组织芯片,经免疫组化染色,统计分析Myo1E蛋白表达的临床意义以及和免疫检查点PD-L1的相关性。结果:Myo1E mRNA和蛋白在PDAC组织中的表达明显高于良性胰腺组织,且与患者预后差有关;Myo1E蛋白表达与肿瘤直径(P=0.004)、淋巴结转移(P<0.001)、侵犯周围组织(P=0.008)、临床分期(P<0.001)相关;生存分析显示,Myo1E蛋白在PDAC组织中表达,是患者预后的独立风险因素(HR:1.686,P=0.010);且与PD-L1的表达具有线性正相关(r=0.388,P<0.001)。结论:PDAC组织中Myo1E是患者预后的独立风险因素,或可成为潜在的免疫治疗靶点。

Objective:Analysis of the expression and clinical significance of myosin 1E(Myo1E)in pancreatic ductal adenocarcinoma(PDAC)tissues.Methods:The expression level of Myo1E mRNA in PDAC tissues and its relationship with prognosis and immune checkpoints were analyzed using the next-generation sequencing data of PDAC in the online cancer genome mapping database,and the clinical significance of Myo1E protein expression and its correlation with the immune checkpoint PD-L1 were analyzed by immunohistochemistry staining using PDAC microarray.Results:The expression of Myo1E mRNA and protein in PDAC tissues was significantly higher than that in benign pancreatic tissues,and was related to the poor prognosis of patients.Myo1E protein expression was associated with tumor diameter(P=0.004),lymph node metastasis(P<0.001),invasion of surrounding tissues(P=0.008),clinical stage(P<0.001).Survival analysis showed that Myo1E protein was expressed in PDAC tissues and was an independent risk factor for patient prognosis(HR:1.686,P=0.010),and was linearly positively correlated with the expression of PD-L1(r=0.388,P<0.001).Conclusion:Myo1E is an independent risk factor for patient prognosis in PDAC tissues and may be a potential target for immunotherapy.