杏鲍菇多糖对小鼠急性化学性肝损伤的抗炎及抗氧化作用

Anti-inflammatory and Antioxidant Effects of Pleurotus eryngii Polysaccharides on Acute Chemical Liver Injury in Mice

为探究杏鲍菇多糖(Pleurotus eryngii polysaccharides,PEP)对CCl_(4)诱导小鼠急性肝损伤的干预作用,首先分析PEP体外清除DPPH自由基及ABTS^(+)自由基的能力,之后将小鼠随机分为对照组、模型组、PEP低、中、高剂量组(100、200、400 mg/kg),连续灌胃干预21 d后,腹腔注射CCl_(4)以诱导小鼠急性肝损伤,最后测定小鼠血清谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)活性、总胆红素(total bilirubin,TBIL)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)含量,分析肝组织丙二醛(malondialdehyde,MDA)含量、过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性并检测肝脏组织形态学变化。结果表明,PEP对ABTS^(+)自由基及DPPH自由基清除率分别可达(70.50±0.63)%和(63.25±0.56)%。与模型组比较,PEP各剂量组肝脏、肾脏及脾脏指数呈不同程度降低。血清ALT、AST活性、TBIL、TC、TG、TNF-α、IL-6含量显著下降,肝组织SOD、CAT、GSH-Px活性显著增高,MDA含量显著下降(P<0.05),且呈剂量依赖性。PEP各剂量组肝组织病理损伤不同程度减轻。因此PEP能够通过抗氧化及抗炎对CCl_(4)所致的小鼠肝损伤发挥保护作用。

To analyze the intervention effect of Pleurotus eryngii polysaccharides(PEP)on carbon tetrachloride(CCl_(4))-induced acute liver injury in mice,the scavenging ability of PEP against DPPH free radicals and ABTS^(+)free radicals in vitro was analyzed.Then,the mice were randomized into the control group,model group,low-,medium-,and high-dose PEP groups(100,200,400 mg/kg).After continuous gavage intervention for 21 d,CCl_(4) was injected intraperitoneally to induce acute liver injury in mice.Finally,the activities of serum alanine transaminase(ALT)and aspartate transaminase(AST),and the contents of total bilirubin(TBIL),total cholesterol(TC),triglyceride(TG),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were measured,the malondialdehyde(MDA)content and the activities of catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the liver tissues were analyzed,and the morphological changes in the liver tissues were detected.The results showed that the scavenging rates of PEP against ABTS^(+)free radicals and DPPH free radicals were(70.50±0.63)% and(63.25±0.56)%,respectively.Compared with the model group,the liver,kidney,and spleen indexes of each PEP group decreased to varying degrees.The serum levels of ALT,AST,TBIL,TC,TG,TNF-α and IL-6 decreased significantly,the activities of SOD,CAT,and GSH-Px in the liver tissues significantly increased,and the content of MDA decreased significantly(P<0.05),in a dose-dependent manner.Pathological damage of liver tissues in each of the PEP groups was alleviated to varying degrees.Therefore,PEP could play a protective role in CCl4-induced liver injury in mice through anti-oxidant and anti-inflammatory effects.