超高效液相色谱-串联质谱法测定孜然中3种硒代氨基酸含量

Determination of 3 kinds of selenoamino acids content in Cuminum cyminum L.by ultra performance liquid chromatography-tandem mass spectrometry

目的采用超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS),对新疆不同地区的孜然进行硒代氨基酸含量分析。方法样品采用水提取法进行提取,以0.1%的乙腈(A)和水(B)二元流动相体系、多反应监测模式进行监测,基质匹配外标法进行定量,对硒代胱氨酸、硒代蛋氨酸和Se-(甲基)硒基-L-半胱氨酸等3种硒代氨基酸进行色谱分离、测定。结果全疆和东疆的3种硒代氨基酸总含量分布较为接近,且东疆总量均值2.635μg/kg与全疆2.705μg/kg接近,北疆总量均值最小,为2.396μg/kg,南疆总量均值最大,为3.003μg/kg,其中产自吐鲁番的孜然中3种硒代氨基酸含量较高。环节中硒代氨基酸总含量的大小顺序为批发环节>零售超市环节>农贸环节,3种硒代氨基酸中硒代胱氨酸含量最多,占比最大。结论本研究采用水提法以及UPLC-MS/MS分析3种硒代氨基酸测定结果准确且操作便捷。结果分析各含量显示同一区域内所产孜然中硒代氨基酸的含量和质量仍可能呈现较大差异,在不同环节下的各种物质含量有明显差异,其中批发环节保持了硒代氨基酸的稳定性,3种硒代氨基酸含量较高。以期对孜然中硒代氨基酸高效测定提供数据支持。

Objective To analy the content of selenoamino acids in Cuminum cyminum L.from different regions of Xinjiang by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Using the water extraction method,a binary mobile phase system consisting of 0.1%acetonitrile(A)and water(B),and multiple reaction monitoring mode for detection,matrix-matched external standard method for quantification,chromatographic separation and determination were performed for 3 kinds of selenoamino acids:Selenocysteine,selenomethionine,and Se-(Methyl)selenyl-L-cysteine.Results The total content of the 3 kinds of selenide amino acids in the whole Xinjiang and the eastern Xinjiang was relatively similar,and the average amount of the eastern Xinjiang was 2.635μg/kg was close to the average amount of the total amount of the whole Xinjiang of 2.705μg/kg.The total amount of the northern Xinjiang was the smallest(2.396μg/kg),and the total amount of the southern Xinjiang was the largest(3.003μg/kg).Among them,3 kinds of selenium amino acid content was high in Cuminum cyminum L.from Turpan.The total content of selenoamino acids in the different links was in the order of wholesale links>retail supermarket links>agricultural market links,with selenocysteine being the most abundant among the 3 kinds of selenoamino acids,having the largest proportion.Conclusion This study employs water extraction and UPLC-MS/MS analysis for the accurate and convenient determination of 3 kinds of selenoamino acids.The results show that within the same region,the content and quality of selenoamino acids in Cuminum cyminum L.can still vary significantly.There are also clear differences in the content of various substances at different stages,with the wholesale stage maintaining the stability of selenoamino acids,and the content of the 3 kinds of selenoamino acids being relatively high.The aim is to provide data support for the efficient determination of selenoamino acids in Cuminum cyminum L..