丝瓜子房培养类胚体发生途径诱导研究

Induction Study on the Pathway of Embryoid Formation in Ovary Culture of Luffa ssp.

[目的]以丝瓜(Luffa ssp.)品种“徐绿一号”的未授粉子房为试验材料,分析不同因素对类胚体形成的影响。[方法]设置L 16(215)正交试验分析子房发育阶段、4℃预冷处理、35℃黑暗热激处理、TDZ、6-BA、ABA、NAA、2,4-D对丝瓜离体子房培养获得类胚体的影响。[结果]不同因素对子房片的发育过程具有不同的效果,其中采集当天开放的雌花、4℃预冷处理、在MS培养基中添加0.04 mg/L TDZ对子房片的类胚体形成具有促进作用,添加0.04 mg/L 2,4-D对丝瓜子房类胚体形成具有抑制作用,但可以促进子房片生根;另外预冷处理与TDZ、TDZ与雌花采集时间、4℃预冷和ABA的两两交互作用对丝瓜类胚体的形成也具有显著影响。[结论]有效形成丝瓜子房类胚体途径:当天采集子房+4℃预冷处理+0.04 mg/L TDZ。

[Objective]To analyze the effects of different factors on embryoid formation using unpollinated ovaries of Luffa ssp.cultivar‘Xulv No.1’as experimental material.[Method]An L 16(215)orthogonal test was set up to analyze the effects of ovary collection time,4℃cold pretreatment,35℃dark heat shock treatment,TDZ hormone,6-BA hormone,ABA hormone,NAA hormone and 2,4-D hormone on embryoid formation in vitro cultured Luffa ssp.ovaries.[Result]Different factors had different effects on the development process of ovary slices.Among them,collecting female flowers that opened on the same day and adding TDZ hormone to MS medium had a promoting effect on embryoid formation in ovary slices.However,2,4-D hormone had an inhibitory effect on embryoid formation in Luffa ssp.ovaries but could promote rooting of ovary slices.In addition,the interaction between 4℃cold pretreatment and TDZ hormone and between TDZ hormone and female flower collection time also had a significant effect on embryoid formation in Luffa ssp..[Conclusion]The effective way to form embryoids from Luffa ssp.ovaries is:collecting ovaries on day of flowering+4℃cold pretreatment+0.04 mg/L TDZ hormone induction in vitro.