棉田不同菌型棉蚜体内微生物多样性分析

Analyze the microbial diversity of cotton aphids with different bacterial types in cotton fields

【目的】研究不同菌型棉蚜体内微生物种类与丰度的差异。【方法】通过HiSeq平台对不同菌型棉田棉蚜体内共生菌的16S rRNA基因V3~V4区进行高通量测序,分析绿盲蝽体内共生菌的种类与多样性。【结果】对照组与沙雷氏菌型、汉密尔顿菌型棉蚜的优势菌门均为变形菌门,相对物种丰度分别占97.42%、95.55%和92.78%。对照组与试验组的优势菌科均为肠杆菌科,但相对丰度有所差异,相对丰度分别占96.14%、81.285%和84.22%。汉密尔顿菌型棉蚜与沙雷氏菌型棉蚜其体内汉密尔顿菌属与沙雷氏菌属微生物丰度与对照组相比显著升高,分别占比77.40%和12.04%。【结论】棉田中含有沙雷氏菌与汉密尔顿菌的棉蚜其体内微生物丰度受到显著影响,其体内汉密尔顿菌属与沙雷氏菌属相对丰度显著上升。

【Objective】To explore the differences of microbial species and abundance in different types of Aphis gossypii Glove.【Methods】The V3-V4 region of 16SrRNA gene of symbiotic bacteria in different types of A.gossypii in cotton fields was sequenced by HiSeq platform,and the species and diversity of symbiotic bacteria in green bug bugs were analyzed.【Results】The dominant phylum of A.gossypii in the control group,serratia and Hamiltonella A.gossypii was Proteus,and the relative species abundance accounted for 97.42%,95.55%and 92.78%,respectively.The dominant bacteria in the control group and the experimental group were Enterobacteriaceae,but the relative abundance was different,accounting for 96.14%,81.285%and 84.22%,respectively.Compared with the control group,the microbial abundance of Hamiltonella and Serratia increased significantly,accounting for 77.40%and 12.04%,respectively.【Conclusion】The microbial abundance of A.gossypii containing Serratia and Hamiltonella in cotton field is significantly affected,and the relative abundance of Hamiltonella and Serratia increased significantly.