2种不同药性中药激活潜伏HSV-1引起“上火”的对比研究

Comparative study on activation of latent HSV-1 induced"Shanghuo"by two different properties of traditional Chinese medicines

中医理论认为“上火”属于阴阳失衡后的综合临床症状群,已有大量临床实践证明服用温热药性中药可引起“上火”,但是其生物学机制和物质基础有待进一步阐明。该研究利用课题组前期已建立的情志应激诱导潜伏Ⅰ型单纯疱疹病毒(HSV-1)复发的“上火”小鼠模型,以平性中药枸杞子Lycii Fructus和温热性中药人参Ginseng Radix et Rhizoma为研究对象,对比和探讨不同药性中药是否会激活HSV-1诱发“上火”,并探讨相关物质基础。结果显示,与枸杞子不同,人参给药14 d能诱导小鼠HSV-1再激活呈现“上火”症状。进一步,该研究制备了人参不同流分包括多糖富集部位(GS1)、水溶性皂苷富集部位(GS2)和非水溶性皂苷富集部位(GS3),并制备了枸杞子不同富集部位枸杞多糖富集部位(LF1)和枸杞多酚富集部位(LF2)。结果发现,与枸杞子给药结果一致,枸杞子2种富集部位LF1和LF2也不能诱发潜伏HSV-1再复发;但是,人参皂苷富集部位GS2和GS3均可引起HSV-1再激活,其中GS3组病毒激活率为100%,由此推测人参引起HSV-1复发可能与其中所含的皂苷成分有关。该研究从HSV-1潜伏再激活角度解析了药源性“上火”的科学内涵,为温热性中药的合理使用提供依据。

Traditional Chinese medicine(TCM)theory suggests that"Shanghuo"is a comprehensive clinical symptom resulting from an imbalance of Yin and Yang.There is substantial clinical evidence showing that the consumption of warm or hot natured TCM can induce"Shanghuo".However,the pharmacological mechanisms and material basis of this phenomenon require further elucidation.In this study,we utilized a mouse model of"Shanghuo"induced by emotional stress to reactivate latent herpes simplex virus type 1(HSV-1)recurrence,and this mouse model was previously established by our research group.This study investigated whether different properties of TCM would activate HSV-1 and induce"Shanghuo"as well as explored the related material basis.Lycii Fructus with a neutral property,and Ginseng Radix et Rhizoma with a warm or hot property were used as research objects.Experimental results showed that unlike Lycii Fructus,administration of Ginseng Radix et Rhizoma for 14 days induced symptoms of"Shanghuo"and reactivated HSV-1 in mice.Furthermore,the study prepared different fractions of Ginseng Radix et Rhizoma,including polysaccharide-enriched fraction(GS1),water-soluble saponin-enriched fraction(GS2),and non-water-soluble saponin-enriched fraction(GS3).Similarly,Lycii Fructus was fractionated into different components,including polysaccharide-enriched fraction(LF1)and polyphenols-enriched fraction(LF2).Consistent with the results of Lycii Fructus administration,neither LF1 nor LF2 induced latent HSV-1 reactivation.However,both GS2 and GS3 fractions of Ginseng Radix et Rhizoma could induce HSV-1 reactivation,with the virus activation rate reaching 100%in the GS3 group.Therefore,it is speculated that the recurrence of HSV-1 is induced by saponins components of Ginseng Radix et Rhizoma.This study elucidates the scientific significance of medicine-induced"Shanghuo"from the perspective of HSV-1 latent reactivation,providing a basis for the rational use of warm or hot natured TCM.