2014-2021年甘肃省柯萨奇病毒A10型VP1区基因特征分析

Genetic characteristics of VP1 region of coxsackievirus A10 in Gansu,2014–2021

目的了解2014—2021年甘肃省柯萨奇病毒A10(Cox A10)的VP1区基因特征及分子流行病学特征。方法对甘肃省59条Cox A10的VP1区序列,使用MEGA11等生物信息分析软件,对这些病毒序列的核苷酸和氨基酸相似度进行了统计分析,并构建了VP1区序列的系统发育树。结果2014—2021年甘肃省流行的Cox A10均为C基因型,分属于C1和C2两个亚型,在亚型中又可划分为7个不同分支,在VP1区表现出9种特征性的氨基酸突变。结论2014—2021年甘肃省Cox A10出现多个流行变异株。因此,加强Cox A10的监测工作,研究其基因变异与临床表现之间的关系显得尤为重要,此外,通过VP1区的分子进化和氨基酸突变分析,可以有效追溯流行株并进行精确分类,为传统流行病学调查提供有力补充。

Objective To understand the genetic characteristics and molecular epidemiological characteristics of the VP1 region of coxsackievirus A10(Cox A10)in Gansu province from 2014 to 2021.Methods Bioinformatics analysis software MEGA11 and others were used to analyze the nucleotide and amino acid similarities of the VP1 region sequences of 59 Cox A10 strains isolated in Gansu,and a phylogenetic tree of the VP1 region sequences was constructed.Results The Cox A10 strains isolated in Gansu from 2014 to 2021 all belonged to genotype C,further dividing into two subtypes,C1 and C2,and into 7 different branches,and 9 characteristic amino acid mutations were found in the VP1 region.Conclusion Multiple variants of Cox A10 emerged in Gansu from 2014 to 2021.Therefore,it is important to strengthen the surveillance for Cox A10,understand the relationship between genetic variation of CoxA10 and clinical manifestation of Cox A10 infection.In addition,the analysis on molecular evolution and amino acid mutation of the VP1 region of Cox A10 can facilitate the epidemic strain tracing and precise classification,providing a strong supplement for traditional epidemiological investigation.