FANCF/BRCA1蛋白表达与急性髓系白血病患者治疗应答的关系

Relationship between FANCF/BRCA1 protein expression and treatment response in patients with acute myeloid leukemia

目的 分析FANCF/BRCA1蛋白表达与急性髓系白血病(AML)患者治疗应答的关系。方法 选取2018年2月—2022年1月秦皇岛市第一医院120例AML患者(观察组),以48例行骨髓活检的非恶性血液疾病患者为对照组。提取所有研究对象骨髓液单个核细胞,采用免疫印迹法半定量分析FANCF/BRCA1蛋白表达,比较观察组和对照组FANCF/BRCA1蛋白表达差异。对AML患者进行诱导治疗,记录其完全缓解率,分析FANCF/BRCA1蛋白表达与患者治疗应答的关系。结果 观察组FANCF/BRCA1蛋白表达水平低于对照组(P<0.001)。120例AML患者中,FANCF/BRCA1蛋白高表达(≥0.79)62例、低表达(<0.79)58例。FANCF/BRCA1蛋白表达与AML患者血红蛋白、血小板计数有关(P<0.05)。FANCF/BRCA1蛋白表达与AML患者治疗应答呈正相关(r=0.548,P<0.05);FANCF/BRCA1蛋白高表达组治疗后完全缓解率(82.26%)高于FANCF/BRCA1蛋白低表达组(56.90%)(P<0.05)。结论 FANCF/BRCA1蛋白表达与AML患者治疗应答呈正相关,可用于评估患者治疗反应。

Objective To analyze the relationship between FANCF/BRCA1 protein expression and treatment response in patients with acute myeloid leukemia(AML).Methods Totally,120 patients with AML in the First Hospital of Qinhuangdao from February 2018 to January 2022(observation group)were enrolled,and 48 patients with non-malignant blood diseases who underwent bone marrow biopsy were enrolled as control group.The mononuclear cells in the bone marrowfluid of all the subjects were extracted,the FANCF/BRCA1 protein expression was analyzed semi-quantitatively by western blotting,and the difference of FANCF/BRCA1 protein expression between observation group and control group was compared.The patients with AML were treated with induction therapy,the complete remission rate was recorded,and the relationship between FANCF/BRCA1 protein expression and therapeutic response was analyzed.Results The expression of FANCF/BRCA1 protein in observation group was lower than that in control group(P<0.001).Among the 120 AML patients,62 cases had high expression of FANCF/BRCA1 protein(≥0.79),and 58 cases had low expression(<0.79).The expression of FANCF/BRCA1 protein was correlated with hemoglobin and platelet count in AML patients(P<0.05).The expression of FANCF/BRCA1 protein was positively correlated with the therapeutic response of AML patients(r=0.548,P<0.05).The complete response rate of FANCF/BRCA1 high expression group(82.26%)was higher than that of FANCF/BRCA1 low expression group(56.90%)(P<0.05).Conclusions The expression of FANCF/BRCA1 protein is positively correlated with the therapeutic response of AML patients,which can be used to evaluate the therapeutic response of patients.