mfat-1转基因小鼠通过促进神经干细胞增殖修复缺氧缺血性脑损伤

Mfat-1 transgenic mice participate in the repair of hypoxic-ischemic brain injury by promoting the proliferation of neural stem cells

目的:验证mfat-1转基因小鼠是否通过促进成体神经干细胞增殖修复缺氧缺血性脑损伤。方法:在体外实验中,分离并培养mfat-1转基因小鼠和同窝阴性鼠的成体神经干细胞,进行氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)造模,检测神经干细胞增殖情况;在体内实验中,对mfat-1转基因小鼠及其同窝阴性对照小鼠进行缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)造模,检测成体神经干细胞的增殖能力。结果:成功构建HIBD小鼠模型和神经干细胞体外培养体系。mfat-1转基因小鼠来源的成体神经干细胞的增殖能力较野生型小鼠来源的成体神经干细胞更强;与野生型小鼠比较,mfat-1转基因小鼠神经干细胞增殖能力显著增强且行为学改善。结论:mfat-1转基因小鼠通过促进神经干细胞增殖修复小鼠HIBD。

Objective:To verify whether mfat-1 transgenic mice participate in the repair of hypoxic-ischemic brain damage by promoting the proliferation of adult neural stem cells.Methods:In vitro experiments,adult neural stem cells from wild-type and mfat-1 transgenic mice were isolated and cultured,subjected to oxygen-glucose deprivation/reoxygenation(OGD/R)modeling,and the proliferation of neural stem cells was assessed;in vivo experiments,mfat-1 transgenic mice and their littermates were subjected to hypoxic-ischemic brain damage(HIBD)modeling,and the proliferation capacity of adult neural stem cells was evaluated.Results:The mouse HIBD model and the neural stem cell in vitro culture system were established successfully.The proliferation ability of adult neural stem cells derived from mfat-1 transgenic mice was higher than that from wild-type mice;mfat-1 transgenic mice showed significantly enhanced proliferation capacity of neural stem cells compared to wild-type mice and displayed better behavioral performance.Conclusion:mfat-1 transgenic mice participate in the repair of HIBD by promoting the proliferation of mouse neural stem cells.