莱氏绿僵菌与常见化学农药的相容性研究

Compatibility of Metarhizium rileyi with Common Chemical Pesticides

为明确莱氏绿僵菌Nr5772菌株与常见化学农药的相容性,本研究采用平板法测定了5种杀菌剂(苯醚甲环唑、甲基硫菌灵、戊唑醇、嘧菌酯、吡唑醚菌酯)和5种杀虫剂(吡虫啉、联苯菊酯、啶虫脒、阿维菌素、毒死蜱)对莱氏绿僵菌菌丝生长的影响。结果表明,5种杀菌剂几乎完全抑制莱氏绿僵菌的生长,相容性极差。杀虫剂联苯菊酯EC_(50)为12926.61 mg/L,与莱氏绿僵菌的相容性最好;吡虫啉、阿维菌素、啶虫脒EC_(50)分别为724.21、264.60、158.49 mg/L,远高于田间常规使用剂量,与莱氏绿僵菌也具有良好的相容性;毒死蜱的EC_(50)为283.38 mg/L,接近其田间常规使用剂量,对莱氏绿僵菌具有较强的抑制作用。综上,在本试验条件下,苯醚甲环唑等5种杀菌剂完全不能与莱氏绿僵菌混用,联苯菊酯、啶虫脒、阿维菌素和吡虫啉4种杀虫剂可与莱氏绿僵菌混用。

To evaluate the compatibility of Metarhizium rileyi Nr5772 with common chemical pesticides,five fungicides(difenoconazole,thiophanate-methyl,tebuconazole,azoxystrobin and pyraclostrobine)and five insecticides(imidacloprid,bifenthrin,acetamiprid,avermectin and chlorpyrifos)were used to study their effects on mycelium growth of M.rileyi by growth rate method.The results showed that the five fungicides almost completely inhibited the mycelium growth of M.rileyi,indicating the compatibility extremely poor.The EC_(50)of bifenthrin against M.rileyi was 12926.61 mg/L showing the best compatibility with M.rileyi.The EC_(50)of acetamiprid,avermectin and imidacloprid were 724.21,264.60 and 158.49 mg/L respectively,which were far above the regular application dosages in field,and indicated that they had excellent compatibility with M.rileyi.The EC_(50)of chlorpyrifos was 283.38 mg/L,nearly to the regular application dosage in field,which showed the stronger inhibitory effect on M.rileyi.In conclusion,under the conditions of this experiment,the five fungicides should not be mixed with M.rileyi,while the four insecticides including bifenthrin,acetamiprid,avermectin and imidacloprid could be mixed with M.rileyi.