一种多功能编织缝合线5-Fu@CHA-TA/PLA的制备和性能研究

Preparation and performance of a multifunctional braided suture 5-Fu@CHA-TA/PLA

为最大限度减少损伤皮肤组织炎症、抑制瘢痕、促进伤口生理性愈合,多功能缝合线是目前临床研究重点。合成单宁酸(TA)增强的酰化透明质酸(CHA),用于负载五氟尿嘧啶(5-Fu)制备功能化缝合线用水凝胶涂层5-Fu@CHA-TA。通过浸涂-紫外光照联用法将7股聚乳酸(PLA)复合纱线为基材编织的缝合线浸渍到5-Fu@CHA-TA水凝胶中,最终制备5-Fu@CHA-TA/PLA多功能缝合线。扫描电镜观察结果显示5-Fu@CHA-TA水凝胶能够均匀涂层到PLA缝合线单丝表面;抗氧化试验、溶血试验及抗菌试验的结果显示,功能化改性的缝合线具有安全稳定的抗氧化性、血液相容性和显著的抗菌效果。缝合线摩擦力试验结果显示制备5-Fu@CHA-TA/PLA多功能缝合线摩擦力明显小于PLA编织缝合线,这有助于减轻受伤组织的摩擦与拖拽。涂层稳定性的测试结果显示,在多次缝合后编织缝隙处依旧存在水凝胶,这一现象确保了缝合线一旦被植入体内,便能够有效发挥其预期的作用。体外细胞相容性测试结果显示,功能化改性的缝合线具有良好的细胞相容性,且释放的5-Fu对成纤维细胞抑制率达65.48%,具有显著抑制组织增生的潜力。研究结果可为功能性缝合线的开发提供新思路。

Multifunctional sutures have become the research hots due to minimize the inflammation of the injured skin tissue,inhibit scar and promote the physiological healing of the wound clinically.In this study,tannic acid(TA)-enhanced acylated hyaluronic acid(CHA)was synthesized and used to prepare functional sutures combined with 5-fluorouracil(5-Fu).The 7-strand poly(lactic acid)(PLA)composite yarn-based braided sutures were impregnated into 5-Fu@CHA-TA hydrogel by dip-coating-ultraviolet photoluminescence coupling method.Finally,5-Fu@CHA-TA/PLA multifunctional sutures were prepared.The SEM results showed that 5-Fu@CHA-TA gel could be uniformly coated on the surface of PLA suture.The in vitro results of antioxidant,hemolysis,and antibacterial tests showed that the functionalized suture had safe and stable antioxidant properties,blood compatibility,and prominent antibacterial effect.Moreover,the friction test showed that the friction of 5-Fu@CHA-TA/PLA suture was significantly lower than that of braided PLA suture,which would help to reduce the friction and drag of injured tissue.Tests on the stability of the coating showed that the hydrogel remained on the braided suture after several suturing operations,ensuring that the suture could perform its intended function upon implantation in the body.In addition,the results of cytocompatibility test in vitro show that the functional sutures present good cytocompatibility,and the inhibitory rate of released 5-Fu on fibroblasts reached 65.48%,which has the potential to significantly inhibit tissue proliferation.This research provides a potential direction for developing functional sutures.