支原体肺炎患者靶向NGS检测分析:单纯感染与合并感染的临床特征比较

Targeted NGS Analysis in Mycoplasma Pneumoniae Patients:A Comparative Study of Clinical Characteristics Between Pure Infection and Co-Infection

目的本研究旨在探究支原体肺炎患者中单纯肺炎支原体感染与肺炎支原体合并其他细菌感染的临床特征和实验室检查结果的差异,并分析性别和年龄对感染类型的影响。方法研究纳入2022年6月1日至2024年4月1日于成都大学附属医院住院经临床和肺泡盥洗液靶向NGS检查确诊为支原体肺炎的患者被纳入研究,共104名。患者根据靶向NGS检测结果分为两组:单纯肺炎支原体感染组46例,肺炎支原体合并其他细菌感染组58例。研究收集了患者的人口统计学特征、实验室检查结果以及住院期间的支气管盥洗液靶向NGS检测结果。结果研究发现淋巴细胞计数、中性粒细胞与淋巴细胞比率、丙氨酸氨基转移酶、乳酸脱氢酶水平上,单纯肺炎支原体感染患者与支原体肺炎合并感染患者之间存在显著性差异(均P<0.05)。女性患者的单纯支原体感染率高于男性(P<0.05)。年龄分析显示,年龄较大的患者更倾向于单纯肺炎支原体感染,而年龄较小的患者更常见于支原体肺炎合并其他细菌感染(均P<0.05)。此外,肺炎链球菌是最常见的合并感染病原体,尤其在儿童中更为常见(P<0.05)。结论本研究揭示了支原体肺炎患者中单纯感染与合并感染的临床和实验室特征差异,以及性别和年龄对感染类型的潜在影响。研究结果有助于更好地理解支原体肺炎的感染模式,并可能对临床诊断和治疗提供指导。

Objective This study aimed to explore the clinical characteristics and laboratory test differences between patients with pure Mycoplasma pneumoniae infection and those with Mycoplasma pneumoniae combined with other bacterial infections,as well as to analyze the impact of gender and age on the type of infection.Methods The study included 104 patients admitted to Chengdu University Hospital from June 1,2022,to April 1,2024,who were diagnosed with Mycoplasma pneumoniae by clinical examination and targeted next-generation sequencing(NGS)of bronchoalveolar lavage fluid.Patients were divided into two groups based on the results of targeted NGS:46 cases of pure Mycoplasma pneumoniae infection and 58 cases of Mycoplasma pneumoniae combined with other bacterial infections.The study collected demographic characteristics,laboratory test results,and bronchoalveolar lavage fluid targeted NGS test results during hospitalization.Results The study found significant differences in lymphocyte count,neutrophil-to-lymphocyte ratio,glutamic-pyruvic transaminase(ALT),and lactate dehydrogenase(LDH)levels between patients with pure Mycoplasma pneumoniae infection and those with combined infections(P<0.05).The rate of pure Mycoplasma pneumoniae infection was significantly higher in female patients than in male patients(P<0.05).Age analysis showed that older patients were more inclined to have pure Mycoplasma pneumoniae infection,while younger patients were more commonly found to have Mycoplasma pneumoniae combined with other bacterial infections(all P<0.05).Streptococcus pneumoniae was the most common co-infecting pathogen,especially more common in children(P<0.05).Conclusions This study revealed the clinical and laboratory characteristic differences between pure and combined Mycoplasma pneumoniae infections,as well as the potential impact of gender and age on the type of infection.The findings may provide guidance for better understanding the infection patterns of Mycoplasma pneumoniae and could inform clinical diagnosis and treatment strategies.