不同盐溶液处理对3种野生芨芨草种子萌发特性的影响

Effects of different types of salt treatments on the germination characteristics of three wild Achnatherum splendens seeds

【目的】探讨芨芨草种子萌发对不同类型盐胁迫的响应。【方法】以3个不同地区(编号分别为YL、BT和ZY)采集的芨芨草种子为材料,测定不同中性盐处理下其种子的萌发特性,利用隶属函数法对种子萌发期的耐盐性进行了综合评价,筛选萌发期耐盐性较强的种质资源。【结果】低浓度Na Cl对BT芨芨草萌发具有促进作用,高浓度Na Cl对ZY、YL和BT芨芨草萌发具有抑制作用,Na2SO4对3种芨芨草的胚根生长均具有抑制作用。随着Na Cl、Na2SO4和混合盐3种盐溶液浓度的逐渐增加,3种芨芨草种子的发芽率、发芽势、发芽指数、活力指数逐渐降低,胚芽长和胚根长逐渐减小。通过隶属函数法进行综合评价,3种盐胁迫处理下芨芨草种子耐盐性强弱依次为:(ZY-混合盐)>(BT-Na Cl)>(YL-混合盐)>(YL-Na2SO4)>(ZY-Na2SO4)>(ZY-Na Cl)>(BT-混合盐)>(BT-Na2SO4)>(YL-Na Cl)。【结论】ZY和YL芨芨草对混合盐耐受性最强,对Na Cl耐受性最差。BT芨芨草对Na Cl耐受性最强,对Na2SO4耐受性最差。

【Objective】Achnatherum splendens seeds collected from three different regions were used as materials to study the effects of different neutral salts on the germination characteristics of their seeds in order to explore the response of Achnatherum splendens seed germination to different types of salt stress.【Method】The membership function method was used to comprehensively evaluate the salt tolerance of Achnatherum splendens seeds at germination stage in three different regions,and to screen out germplasm resources with stronger salt tolerance at germination stage,in order to provide theoretical basis for Achnatherum splendens planting and new variety breeding in saline-alkali areas.【Result】The results showed that low concentration of NaCl could promote the germination of BT splendens,high concentration of NaCl could inhibit the germination of ZY,YL and BT splendens,and Na2SO4 had inhibitory effects on the radicle growth of three splendens.With the increasing concentrations of NaCl,Na2SO4 and mixed salts,the germination rate,germination potential,germination index and vigor index of the three Achnatherum splendens seeds gradually decreased,and the germ length and radicle length decreased gradually.According to the comprehensive evaluation by membership function method,the salt tolerance of Splendens seeds under the three salt stress treatments is as follows:(Zy-mixed salt)>(BT-NaCl)>(YL-mixed salt)>(YL-Na2SO4)>(ZY-Na2SO4)>(ZY-NaCl)>(BT-mixed salt)>(BT-Na2SO4)>(YL-NaCl).【Conclusion】ZY and YL Achnatherum splendens had the strongest tolerance to mixed salt and the worst tolerance to NaCl.BT splendens had the strongest tolerance to NaCl and the worst tolerance to Na2SO4.