miR-127-3p下调MAPK p38抑制三阴性乳腺癌细胞转移

miR-127-3p inhibited metastasis of triple-negative breast cancer cells by down-regulating MAPK p38

[目的]探究miR-127-3p与MAPK p38对三阴性乳腺癌细胞转移的影响。[方法]采用免疫组化实验分析乳腺癌组织和癌旁组织中的MAPK p38表达水平。将人三阴性乳腺癌细胞MDA-MB-231细胞分为4组:miR NC组、miR-127-3p mimic组、si NC组和si MAPK p38组。通过CCK-8试剂检测细胞生长能力。通过实时荧光定量PCR实验检测miR-127-3p和MAPK p38表达水平。通过Transwell实验分析MDA-MB-231细胞迁移能力。通过蛋白免疫印迹实验分析MAPK p38表达水平。通过流式细胞术检测MDA-MB-231细胞凋亡率。[结果]与癌旁组织样本比较,乳腺癌组织样本miR-127-3p表达水平降低(0.88±0.09 vs 0.23±0.09,P<0.05),而MAPK p38的表达水平增加。细胞实验结果显示,与miR NC组比较,miR-127-3p mimic组MDA-MB-231细胞的增殖能力和转移能力明显降低,凋亡率增加(6.02±0.08 vs 13.13±0.29,P<0.05)。与si NC组比较,si MAPK p38组MDA-MB-231细胞的增殖能力和转移能力明显降低,凋亡率增加(5.03±0.11 vs 15.03±0.92,P<0.05)。荧光素酶报告基因结果显示,miR-127-3p能够靶向调控MAPK p38表达(0.91±0.22 vs 0.25±0.13,P<0.05)。[结论]上调miR-127-3p表达能够抑制MDA-MB-231细胞的增殖能力和转移能力,并能促进MDA-MB-231细胞凋亡。此外,miR-127-3p对MDA-MB-231细胞的这一作用与靶向抑制MAPK p38表达有关。

[Objective]To investigate the effects of miR-127-3p and MAPK p38 on metastasis of triple-negative breast cancer cells.[Method]The expression of p38 MAPK in breast cancer tissues and adjacent tissues was analyzed by immunohistochemistry.Human triple-negative breast cancer cell MDA-MB-231 cells were divided into four groups:miR NC group,miR-127-3p mimic group,si NC group and si MAPK p38 group.Cell growth ability was detected by CCK-8 reagent.The expression levels of miR-127-3p and MAPK p38 were detected by real-time fluorescence quantitative PCR.Cell migration ability was analyzed by Transwell assay.The expression level of MAPK p38 was analyzed by Western Blot.The apoptosis rate was analyzed by flow cytometry.[Result]Compared with the adjacent tissues,the expression level of miR-127-3p in breast cancer tissues was decreased(7.68±0.09 vs 0.23±0.09,P<0.05),while the expression level of MAPK p38 was increased.Cell experiments showed that compared with the miR NC group,the proliferation and metastasis ability of MDA-MB-231 cells in the miR-127-3p mimic group were significantly decreased,and the apoptosis rate was increased(6.02±0.08 vs 13.13±0.29,P<0.05).Compared with the si NC group,the proliferation and metastasis ability of MDA-MB-231cells in the si MAPK p38 group were significantly decreased,and the apoptosis rate was increased(5.03±0.11 vs 15.03±0.92,P<0.05).Luciferase reporter gene results showed that miR-127-3p could target and regulate the expression of MAPK p38(0.91±0.22 vs 0.25±0.13,P<0.05).[Conclusion]Up-regulation of miR-127-3p expression can inhibit the proliferation and metastasis of MDA-MB-231 cells,and promote the apoptosis of MDA-MB-231 cells.In addition,the effect of miR-127-3p on MDA-MB-231 cells was related to the targeted inhibition of MAPK p38 expression.