载物台原位培养器内部传热特性仿真研究

Simulation Research on Heat Transfer Characteristics in Miniaturized Stage Top Cell Culture Device

载物台原位细胞培养和观测装置对于研究细胞的实时动态行为具有重要作用,而目前对于该类微型装置内部的传热特性研究尚不全面。本文设计了这一种具有对称循环风扇的微型培养器,建立了多物理场仿真模型,并对其三维温度分布、气体对流以及水蒸气影响效果等进行了模拟和分析。结果表明,在考虑培养器内水分蒸发效应情况下,总功率60W加热片可在20min内使得内部培养板内孔底部液体升温至37℃,且在考虑水蒸气的传热和传质效应下的平均液温比忽略蒸发时的平均液温降低约1.6℃以上。

The stage top devices for in situ cell culture and observation play an important role in the study of the real-time dynamic behavior of cells,but the research on the heat transfer characteris-tics inside such micro-devices is still incomplete.In this paper,a micro-incubator with symmetrically mounted circulating fans on the two-opposite chamber-walls is designed,and a multi-physical field simulation model is established,and the three-dimensional temperature distribution,gas convection and water vapor effect are simulated and analyzed.The results show that the liquid temperature at the bottom of the inner hole of the cell culture plate can be raised to 37℃within 20 minutes by heating plates with a total power of 6ow,and the average liquid temperature under the consideration of the heat and mass transfer effects of water vapor is more than 1.6℃lower than the average water temperature when evaporation is ignored.