肿瘤靶向近红外荧光染料NIR-T用于清晰显影乳腺癌切缘的探索

Exploration of tumor-targeted near infrared fluorescent dye NIR-T for clearly delineating incisal edge of breast cancer

目的探索肿瘤靶向近红外荧光染料NIR-T显示乳腺肿瘤切缘的准确性。方法首先在BALB/c nude小鼠构建乳腺癌异种移植皮下瘤,瘤内注射染料NIR-T,评价NIR-T显影乳腺肿瘤切缘信噪比,并在荧光信号指引下分别切除和采集肿瘤与瘤旁组织,通过病理分析评价NIR-T显影肿瘤切缘准确性。进一步在新鲜的人乳腺肿瘤离体组织上进行实验,瘤内注射NIR-T,在荧光信号指引下,切除采集肿瘤和瘤旁组织,利用病理分析评价NIR-T显影肿瘤切缘准确性。结果染料NIR-T可清晰显影BALB/c nude小鼠皮下乳腺肿瘤切缘,信噪比可达15,成功引导肿瘤的快速切除,术中病理切片分析证明肿瘤切除干净;染料NIR-T可清晰显影离体的人乳腺肿瘤切缘,且准确性与病理切片分析的匹配度达100%。结论肿瘤靶向近红外荧光染料NIR-T可清晰、准确显影乳腺肿瘤切缘,有助于提升肿瘤切除精度,缩短手术时长。

ObjectiveTo explore the feasibility of the tumor-targeted near infrared(NIR)fluorescent dye NIR-T for clearly and accurately delineating incisal edge of breast cancer.MethodsFirstly,the breast cancer xenograft subcutaneous tumor was established in BALB/c nude mice,and dye NIR-T was administrated intratumorally to evaluate the tumor-to-normal tissue(T/N)ratio of the incisal edge of breast tumor.Under the guidance of fluorescence signals,the tumor and peritumoral tissues were resected and collected respectively,and the accuracy of the tumor incisal edge delineated by NIR-T was evaluated by pathological analysis.Studies were further performed on fresh isolated human breast tumor tissues.After intratumoral injection of NIR-T,tumor and peritumoral tissues were collected with the guidance of fluorescence signals,and the accuracy of breast tumor incisal edge delineated by NIR-T was evaluated by pathological analysis.ResultsDye NIR-T was able to clearly delineate the incisal margin of subcutaneous breast tumor in BALB/c nude mice with a T/N ratio of up to 15,and was used to successfully guide the rapid resection of the tumor,and subsequent intraoperative pathological analysis confirmed that the tumor was absolutely removed;NIR-T could be used to clearly delineate the resection margin of breast tumors and its precision was determined to be 100%via pathological analysis.ConclusionThe tumor-targeted NIR fluorescent dye NIR-T was able to clearly and accurately delineate the resection margin of breast tumors,which would promote the precision of tumor resection and shorten the operation time.