五种中药方药对小鼠急性肺损伤的治疗作用

Therapeutic effects of five traditional Chinese medicine prescriptions on acute lung injury in mice

目的探讨新冠治疗中使用的五种中药方药—湿毒郁肺方、内闭外脱方、气阴两虚方、银翘藿朴方、荆防藿朴方在小鼠急性肺损伤模型中的治疗效果及其潜在作用机制。方法实验小鼠分为7组,分别为正常组、急性肺损伤组以及五个中药方药治疗组。采用脂多糖滴鼻法建立小鼠的急性肺损伤模型。治疗组小鼠分别予以相应的中药方剂进行灌胃治疗,而急性肺损伤组和对照组给予等量0.9%氯化钠注射液。治疗1周后,处死小鼠,收集肺组织进行苏木精-伊红染色和q-PCR检测,以评估肺组织损伤及炎症因子表达情况。结果与正常对照组比较,急性肺损伤组小鼠的肺组织出现明显的病理损伤,五种中药方药治疗组的小鼠在苏木精-伊红染色中显示出肺组织病理损伤有明显减轻。通过q-PCR分析发现,与急性肺损伤组比较,五种中药方药治疗组IL-6 mRNA含量均有所降低,差异具有统计学意义(P<0.05)。结论五种中药方药均能有效减轻小鼠急性肺损伤症状,其作用机制可能与其对炎症因子表达的抑制作用有关。这些结果为中药在新冠肺炎及相关肺部疾病治疗中的应用提供一定的实验依据,并展示中药在治疗急性肺损伤中的潜在价值。

Objective To explore the treatment effects and potential mechanisms of five traditional Chinese medicine prescriptions including Shidu Yufei formula,Neibi Waidao formula,Qiyin Liangxu formula,Yin Qiao Huo Po formula and Jing Fang Huo Po formula,used in the treatment of COVID-19,on a mouse model of acute lung injury.Methods Experimental mice were divided into seven groups:normal control,acute lung injury model,and five traditional Chinese medicine treatment groups.The acute lung injury model in mice was established using lipopolysaccharide nasal drops.Mice in the treatment groups were administered the respective Chinese medicine prescriptions via gavage,while the model and control groups received an equivalent volume of saline.One week after treatment,the mice were euthanized,and lung tissues were collected for hematoxylin-eosin staining and q-PCR analysis to evaluate lung tissue damage and the expression of inflammatory factors.Results Compared to the normal control group,significant pathological damage was observed in the lung tissues of the model group.The mice in the traditional Chinese medicine treatment groups showed a significant reduction in lung tissue pathology as indicated by hematoxylin-eosin staining.q-PCR analysis revealed that,compared to the acute lung injury model group,all five traditional Chinese medicine treatment groups showed a decrease in IL-6 mRNA levels and an increase in IL-10 levels,with statistically significant differences(P<0.05).Conclusion This study demonstrates that the five traditional Chinese medicine prescriptions effectively alleviate symptoms of acute lung injury.The mechanism may be related to their inhibitory effects on the expression of inflammatory factors.These results provide experimental evidence for the application of traditional Chinese medicine in the treatment of COVID-19 and related pulmonary diseases,demonstrating the potential value of traditional Chinese medicine in treating acute lung injury.