肝性脑病造模方法与临床吻合度分析

Analysis of hepatic encephalopathy modeling methods and clinical match

分析现有的肝性脑病动物模型方法与临床吻合度,以期为建立更符合中西医临床病症特点的动物模型提供思路。在中国知网、万方数据库,以“肝性脑病”“肝昏迷”“动物”“动物模型”为关键词,在Pubmed数据库,以“hepatic encephalopathy”“hepatic coma”“animal model”“animal”为关键词进行检索,整理有具体动物模型制备方法的有效文献,根据不同造模因素和方式进行整理、分类,概括造模方法、特点,依据肝性脑病中西医临床病症特点对动物模型的临床吻合情况进行赋值、分析。有效文献128篇,共11种动物模型,包含15种不同的造模方法,西医吻合度最高的是CCl4油溶液腹腔注射+灌胃+氯化铵腹腔注射模型(吻合度92%),中医吻合度最高的是CCL4油溶液腹腔注射+乙醇水溶液自饮模型(吻合度65%),但缺乏中西医吻合度均较高的肝性脑病动物模型。目前缺乏中西医病症特点结合的肝性脑病动物模型,结合中西医病症特点进行改进和完善,有望为肝性脑病的机制研究和临床诊治提供思路。

To analyze the existing method for obtaining animal models of hepatic encephalopathy and their clinical characteristics with a view to providing ideas for the establishment of animal models that are more in line with the clinical characteristics of Chinese and Western medicine.To search the Chinese Knowledge Network and Wanfang database,the keywords“hepatic encephalopathy”,“hepatic coma”,“animal”,and“animal model”were used.For the Pubmed database,“hepatic encephalopathy”,“hepatic coma”,“animal model”,and“animal”were used as search keywords.The literature on effective method of specific animal model preparation was collated and classified according to different modeling factors and method.The modeling method and characteristics were summarized,and the degree to which the animal models shared the clinical characteristics and symptoms of hepatic encephalopathy according to Chinese and Western medicine was assessed.The clinical match of the animal model was assigned and analyzed according to the clinical symptoms of hepatic encephalopathy in Chinese and Western medicine.There were 128 valid literature articles featuring 11 animal models obtained using 15 different modeling method.The highest degree of agreement with Western medicine was the CCl_4 oil solution intraperitoneal injection+gavage+ammonium chloride intraperitoneal injection model(92%),and the highest degree of agreement with traditional Chinese medicine was the CCl_4 oil solution intraperitoneal injection+ethanol aqueous solution self-drinking model(65%),but both lacked a combination of Chinese and Western medicine clinical characteristics.At present,animal models of hepatic encephalopathy that combine the characteristics of Chinese and Western medicine are lacking.Improving and refining the models to encompass the characteristics of both Chinese and Western medicine are expected to provide ideas for mechanism research and advance the clinical diagnosis and treatment of hepatic encephalopathy.