嗅觉通路损伤后OMP变化及白藜芦醇对其表达的影响

The changes of OMP protein after olfactory pathway damage and the effect of resveratrol on it

目的:探究嗅觉通路损伤后嗅觉标志蛋白(OMP)变化及白藜芦醇对其表达的影响。方法:实验动物随机分为正常对照组(对照组),白藜芦醇给药组(给药组),甲醛蓄积组(甲醛组),甲醛蓄积+白藜芦醇给药组(甲醛+给药组)。对照组不做任何处理;给药组和甲醛+给药组于每日上午8时给予白藜芦醇灌胃治疗,灌胃剂量80 mg/(kg·d),连续灌胃30 d;甲醛组和甲醛+给药组于每日上午9时至12时置于染毒室,共30 d。30 d后行食物埋藏实验、嗅觉躲避实验、嗅觉记忆实验检测其嗅觉功能;处死实验动物后取嗅神经,检测其OMP表达。结果:实验过程中动物无丢失,无死亡;食物埋藏实验结果显示:甲醛组找到埋藏食物时间较对照组长,差异有统计学意义(P<0.05);甲醛+给药组食物埋藏时间较对照组长,较甲醛组时间短,差异有统计学意义(P<0.05)。嗅觉躲避实验结果显示:甲醛组在双蒸水小室所待时间较对照组短,差异有统计学意义(P<0.05);甲醛+给药组双蒸水小室时间较对照组短,较甲醛组时间长,差异有统计学意义(P<0.05)。嗅觉记忆实验结果显示:甲醛组在精油小室所待时间较对照组长,差异有统计学意义(P<0.05);甲醛+给药组双蒸水小室时间较对照组长,较甲醛组时间短,差异有统计学意义(P<0.05)。Western印迹结果显示:甲醛组蛋白含量明显下降,与对照组相比差异有统计学意义(P<0.05);甲醛+给药组蛋白含量较对照组降低,较甲醛组相比略有升高,差异有统计学意义(P<0.05)。结论:白藜芦醇对嗅觉通路印迹损伤大鼠行为学变化具有明显的促进作用;甲醛蓄积大鼠嗅神经OMP含量降低,白藜芦醇干预后甲醛蓄积大鼠嗅神经OMP蛋白表达呈现上升趋势。

Objective To investigate the changes of OMP protein and the effects of resveratrol on it after the olfactory imprinting of gaseous formaldehyde.Method The experimental animals were randomly divided into normal control group(referred to as control group),resveratrol administration group(referred to as administration group),formaldehyde accumulation group(referred to as formaldehyde group),formaldehyde accumulation+resveratrol administration group(referred to as formaldehyde+administration group).The control group was not treated at all,the administration group and the formaldehyde+administration group were given resveratrol ga vage treatment at 8 a.m.every day,with a gavage dose of 80 mg/(kg·d)for 30 days,and the formaldehyde group and formaldehyde+administration group were placed in the poisoning room from 9 a.m.to 12 p.m.every day for a total of 30 days.After 30 days,food burial experiments,olfactory avoidance experiments and olfactory memory experiments were performed to detect olfactory function,and olfactory nerves were taken to detect OMP expression after the experimental animals were sacrificed.Results The results of food burial experiment showed that the time for finding and burying food in the formaldehyde group was longer than that in the control group,and the difference was statistically significant(P<0.05),and the time of food burial in the formaldehyde+administration group was longer than that in the control group,and the difference was statistically significant(P<0.05).The results of olfactory avoidance experiment showed that the time spent in the double distilled water chamber in the formaldehyde group was shorter than that in the control group,and the difference was statistically significant(P<0.05),and the time in the formaldehyde+administration group was shorter than that in the control group,and the difference was statistically significant(P<0.05).The results of olfactory memory experiment showed that the formaldehyde group spent a statistically significant time in the essential oil chamber compared with the control group(P<0.05),and the formaldehyde+administration group had a statistically significant difference in the time of the double distilled water chamber compared with the control group and a shorter time than the formaldehyde group(P<0.05).The results of Western blot showed that the histone content of formaldehyde decreased significantly compared with the control group(P<0.05),and the histone content of formaldehyde+administration decreased compared with the control group,and increased slightly compared with the formaldehyde group,and the difference was statistically significant(P<0.05).Conclusion Resveratrol can obviously promote the behavioral changes of rats with olfactory imprinting injury.The content of OMP protein in olfactory nerve of formaldehyde-accumulated rats decreased,and the expression of OMP protein in olfactory nerve of formaldehyde-accumulated rats increased after resveratrol intervention.