SFRP4、HMGB1、DEPDC1B及PD-L1表达在三阴性乳腺癌组织中的临床意义

Expression of SFRP4,HMGB1,DEPDC1B and PD-L1 in Triple Negative Breast Cancer

目的探讨分泌性卷曲相关蛋白4(SFRP4)、高迁移率族蛋白B1(HMGB1)、DEP结构域蛋白1B(DEPDC1B)、程序性死亡蛋白配体-1(PD-L1)在三阴性乳腺癌(TNBC)组织中表达意义。方法选取83例TNBC患者作为研究对象,均于术中采集肿瘤组织及癌旁正常组织,采用免疫组化SP法检测SFRP4、HMGB1、DEPDC1B及PD-L1表达情况,比较肿瘤组织及正常组织内上述表达情况;并分析SFRP4、HMGB1、DEPDC1B及PD-L1表达与患者年龄、绝经状态、肿瘤直径、淋巴结转移、肿瘤分期等病理特征的关系。结果肿瘤组织内SFRP4、HMGB1、DEPDC1B及PD-L1阳性表达率高于正常组织,差异有统计学意义(P<0.05);SFRP4、HMGB1、DEPDC1B及PD-L1阳性表达患者肿瘤直径≥3 cm、临床分期Ⅲ~Ⅳ期、有淋巴结转移占比高于SFRP4、HMGB1、DEPDC1B及PD-L1阴性表达患者,差异有统计学意义(P<0.05)。结论SFRP4、HMGB1、DEPDC1B及PD-L1在TNBC组织内存在较高表达,且高表达与肿瘤分期、淋巴结转移关系密切,可用于指导临床完善治疗方案。

Objective To investigate the expression significance of secreted crimp-associated protein 4(SFRP4),high mobility group protein B1(HMGB1),DEP domain protein 1B(DEPDC1B),programmed death protein ligand-1(PD-L1)in triple negative breast cancer(TNBC)tissues.Methods 83 patients with TNBC were selected as the study objects.Tumor tissues and adjacent normal tissues were collected during the operation.The expression of SFRP4,HMGB1,DEPDC1B and PD-L1 were detected by immunohistochemical SP method.The relationship between the expression of SFRP4,HMGB1,DEPDC1B and PD-L1 and the pathological characteristics of patients such as age,menopause,tumor diameter,lymph node metastasis and tumor stage was analyzed.Results The positive expression rates of SFRP4,HMGB1,DEPDC1B and PD-L1 in tumor tissues were higher than those in normal tissues,and the difference was statistically significant(P<0.05).Patients with positive expression of SFRP4,HMGB1,DEPDC1B and PD-L1 had tumor diameter≥3 cm,clinical stageⅢ~Ⅳ,and the proportion of lymph node metastasis was higher than those with negative expression of SFRP4,HMGB1,DEPDC1B and PD-L1,and the difference was statistically significant(P<0.05).Conclusion SFRP4,HMGB1,DEPDC1B and PD-L1 are highly expressed in TNBC tissues,and the high expression is closely related to tumor stage and lymph node metastasis,which can be used to guide clinical improvement of treatment.