基于学习进阶理论的“微生物的纯培养”实验教学

Teaching the experiment“pure culture of microorganisms”based on the theory of learning progressions

在“微生物的纯培养”实验教学过程中,基于学习进阶理论,以地衣内生菌的分离、纯化和培养为主线任务,引导学生依托文献生成课题,完成对“髓层刮离法”的自主设计和操作,在真实科研情境中发展学生的技术和工程等高阶思维。实验结果表明,“髓层刮离法”在提高分离率和降低污染率方面均要优于传统方法。

In teaching the experiment“pure culture of microorganisms”,based on the theory of learning progressions,we set the separation,purification,and culture of endophytic fungi in lichens as the main task,guide students to generate topics based on literature,complete independent design and operation of the“medulla scraping method”,and develop their high-order thinking in technology and engineering in a real scientific research situation.The experimental results show that the“medulla scraping method”is superior to traditional methods in enhancing separation rate and reducing contamination rate.