番木瓜TCP转录因子鉴定及在果实成熟中的表达

Identification of TCP transcription factors in Carica papaya and their expression during fruit ripening

TCP蛋白是植物特有的转录因子,在植物生长发育、信号转导以及逆境响应等方面发挥着重要作用。该研究利用生物信息学方法对番木瓜TCP基因家族成员进行系统鉴定,并对其进化关系、保守基序、保守结构域、基因结构、蛋白三级结构和其在果实成熟中表达模式进行了相关分析。结果表明,番木瓜TCP基因家族中含有22个成员;TCP基因家族成员可分为2大类:ClassⅠ和ClassⅡ,其中ClassⅡ可细分为CYC/TB1和CIN两个亚类;同一亚组内,TCP蛋白具有相似的保守基序和保守结构域,不同亚组之间具有较大差异,表明不同类型TCP蛋白可能具有独特的生理功能;22个家族成员均有bHLH(basic-helix-loop-helix)保守区,且其基因结构由1~7个外显子和1~6个内含子构成;ClassⅡ亚族基因表达量随着果实的成熟显著下调,推测ClassⅡ亚族成员能够抑制果实成熟。这些结果有助于今后揭示番木瓜TCP基因家族成员的功能。

TCP protein is the plant specific transcription factors,which plays an important role in the aspects of plant growth and development,signal transduction and biochemical stimulation response.In this research,we systematically screened the family members of TCP gene of Carica papaya and analyzed their phylogenetic relationship,conserved motifs,conserved domains,gene structure,protein tertiary structure and expression pattern in fruit maturation by using the bioinformatics methods.The results showed that 22 TCP genes existed in the Carica papaya TCP gene family.TCP gene family could be divided into two classes:ClassⅠand ClassⅡ,and ClassⅡcould be further classified into two subclasses(CYC/TB1 and CIN).Within the same subgroup,TCP proteins had similar conserved motifs and conserved domains,and there were large differences between different subgroups,indicating that different types of TCP proteins might have unique physiological functions.The 22 family members all had bHLH(basic-helix-loop-helix)conserved region and their genetic structure consisted of 1-7 exon and 1-6 introns;Gene expression in ClassⅡsubgroup decreased significantly with fruit ripening,so it was assumed that ClassⅡsubgroup had no effect on fruit ripening or inhibited fruit ripening.The above results would be helpful to reveal the function of TCP gene family members in Carica papaya.