HPLC-MS/MS法测定棉花纤维中6种脱叶剂的残留量

Determination of 6 defoliants in cotton fiber by HPLC-MS/MS method

建立了一种高效液相色谱-串联质谱法(HPLC-MS/MS)测定棉花纤维中噻苯隆、噻节因、敌草隆、脱叶磷、乙烯利、环丙酸酰胺6种脱叶剂残留量的检测方法。超声萃取溶剂采用乙腈和水(体积比1∶1),超声萃取20 min后过滤,采用高效液相-串联质谱(HPLC-MS/MS)分析。该方法的目标物质在一定的浓度范围内具有良好的线性关系,相关系数均大于0.995,6种化合物的平均回收率在60.8%~101.6%,相对标准偏差RSD在2.1%~8.6%,乙烯利、噻节因、脱叶磷的定量限为1.0 mg/kg,环丙酸酰胺、噻苯隆、敌草隆的定量限为0.5 mg/kg。

A high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)method is established to determine six defoliant residues of thidiazuron,dimethipin,diuron,tribufos,ethephon and cyclan⁃ilide in cotton fibers.Ultrasonic extraction is performed using acetonitrile and water(volume ratio 1∶1)as sol⁃vents for 20 min,followed by filtration and analysis using high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS).The average recoveries of the six defoliants are 60.8%-101.6%,the rela⁃tive standard deviations are 2.1%-8.6%,the quantitation limits of ethephon,dimethipin and tribufos phosphorus are 1.0 mg/kg,and the quantitation limits of cyclanilide,thidiazuron and diuron are 0.5 mg/kg.