基于BSA-seq技术定位黄瓜黄绿叶色突变基因

Mapping of cucumber virescent yellow leaf mutant gene based on BSA-seq technology

为明确课题组从地方收集的黄瓜黄绿叶色突变体中黄绿叶色突变基因,本研究利用绿叶(野生型)与黄绿叶色(突变体)黄瓜材料为亲本,配制正反杂交一代及二代分离群体,在分析黄绿叶色突变基因遗传规律的基础上,利用BSA-seq技术对双亲及F 2代叶色极端混池进行测序,筛选黄瓜黄绿叶色突变性状的关联标记及候选基因。结果表明,绿叶色对黄绿叶色为完全显性,黄绿叶色突变基因为隐性基因。突变性状关联分析(SNP-index检测)得到1个与黄绿叶色突变相关的候选区域,位于黄瓜1号染色体上,总长度18484 bp。该候选区域共有118个与黄绿叶色突变相关的SNP位点;候选区域共注释到3个基因,其中CsaV3_1G032820基因为黄瓜黄绿叶色突变最可能相关基因。本研究结果可为黄瓜黄绿叶色突变基因克隆和分子标记辅助育种提供基础。

In order to clarify the virescent yellow leaf mutant gene in cucumber virescent yellow leaf mutant collected by the research group from the local area,this study used green leaf(wild type)and virescent yellow leaf(mutant)cucumber materials as parents to prepare a reciprocal hybrid generation and a second generation separation population.On the basis of analyzing the genetic law of virescent yellow leaf mutant genes,BSA-seq technology was used to sequence the extreme mixing pools of parents and F 2 generations,and to screen the associated markers and candidate genes of cucumber virescent yellow leaf mutant traits.The results showed that the green leaf color was completely dominant to virescent yellow leaf color,and the virescent yellow leaf color mutant gene was a recessive gene.A candidate region associated with virescent yellow leaf color mutation was obtained by SNP-index detection,which was located on chromosome 1 of cucumber,with a total length of 18484 bp.A total of 118 SNP loci associated with virescent yellow leaf color mutation were found in this candidate region.Three genes were annotated in the candidate region,among which CsaV3_1G032820 gene was the most likely related gene of cucumber virescent yellow leaf color mutation.The results of this study can provide a basis for cucumber virescent yellow leaf color mutant gene cloning and molecular marker-assisted breeding.