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氧化铈纳米颗粒引发处理对盐胁迫下辣椒植株生长、生理特性及相关耐盐基因表达的影响

Effects of cerium oxide nanoparticles seed priming on growth and physiological characteristics,and expression of stress resistance genes in pepper plants under salt stress
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摘要 辣椒是一种对盐胁迫敏感的经济作物。已有研究发现,用氧化铈纳米颗粒(CeO_(2)NPs)对植物种子进行引发处理可以提高植物的耐盐能力。本研究旨在分析用不同浓度(0 mmol/L、0.05 mmol/L、0.10 mmol/L、0.20 mmol/L、0.30 mmol/L、0.40 mmol/L和0.50 mmol/L)CeO_(2)NPs对辣椒种子进行引发处理后,辣椒植株在盐胁迫下的生长、抗逆生理及叶绿素荧光参数、矿质元素含量及相关耐盐基因的表达情况。结果表明,用CeO_(2)NPs对辣椒种子进行引发处理可以减轻盐胁迫造成的辣椒植株生长受阻,提高盐胁迫下辣椒植株的总鲜重、总干重,CeO_(2)NPs的最佳使用浓度为0.05 mmol/L。用适宜浓度的CeO_(2)NPs对辣椒种子进行引发处理提高了盐胁迫下辣椒叶片中相关抗氧化酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化氢酶(APX)]的活性、渗透调节物质(可溶性糖、可溶性蛋白质和脯氨酸)含量,降低了丙二醛含量。此外,用适宜浓度的CeO_(2)NPs对辣椒种子进行引发处理上调了辣椒叶片中5个耐盐相关基因(CaAnn9、CaNCED3、CabZIP25、CaSBP12和CaOSM1)的相对表达量。综上,用适宜浓度的CeO_(2)NPs对辣椒种子进行引发处理通过促进辣椒叶片抗氧化酶系统的建立与渗透调节物质的积累来维持盐胁迫下辣椒叶片的氧化还原稳态与渗透平衡,同时通过上调辣椒叶片中相关耐盐基因的相对表达量来增强辣椒植株对盐胁迫的耐受性。 Capsicum annuum L.is an economic crop sensitive to salinization.Seed priming treatment with cerium oxide nanoparticles(CeO_(2)NPs)can improve the salinization tolerance of plants.In this study,we investigated the effects of seed priming treatment with different concentrations(including 0 mmol/L,0.05 mmol/L,0.10 mmol/L,0.20 mmol/L,0.30 mmol/L,0.40 mmol/L,and 0.50 mmol/L)of CeO_(2)NPs on growth,stress resistance physiology,chlorophyll fluorescence parameters,mineral element content and expression of salinization-tolerant genes of C.annuum seedlings under salt stress.Our results showed that seed priming treatment with CeO_(2)NPs could alleviate the growth inhibition of pepper plants caused by salt stress,and increase the total fresh and dry weight under salt stress.The optimum concentration of CeO_(2)NPs was 0.05 mmol/L.Seed priming treatment with appropriate concentration of CeO_(2)NPs increased the activities of superoxide dismutase(SOD),catalase(CAT)and ascorbate catalase(APX)and the contents of osmotic adjustment substances(soluble sugar,soluble protein and proline),but decreased the content of malondialdehyde(MDA)in the leaves of C.annuum seedlings under salt stress.In addition,seed priming treatment with appropriate concentration of CeO_(2)NPs enhanced the relative expression levels of five related salinization-stress resistance genes(CaAnn9,CaNCED3,CabZIP25,CaSBP12 and CaOSM1).In summary,the priming treatment of pepper seeds with appropriate concentration of CeO_(2)NPs can maintain the redox homeostasis and osmotic balance under salt stress by promoting the establishment of antioxidant enzyme system and the accumulation of osmotic adjustment substances in pepper leaves,and enhance the tolerance of pepper plants to salt stress by up-regulating the expression of related salt-tolerant genes in pepper leaves.
作者 秦中维 魏茜雅 梁腊梅 林欣琪 李映志 QIN Zhongwei;WEI Qianya;LIANG Lamei;LIN Xinqi;LI Yingzhi(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang 524088,China)
出处 《江苏农业学报》 CSCD 北大核心 2024年第9期1719-1730,共12页 Jiangsu Journal of Agricultural Sciences
基金 广东省科技厅科技计划项目(2016A020210116、2012A020602051) 广东海洋大学创新强校工程科研项目(GDOU2016050256、GDOU2013050217)。
关键词 氧化铈纳米颗粒 种子引发 盐胁迫 辣椒 cerium oxide nanoparticles seed priming salt stress pepper
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