滨州冬枣园区根围丛枝菌根真菌多样性研究

Arbuscular mycorrhizal fungi diversity in the rhizosphere of Dongzao jujube trees(Ziziphus jujuba Mill.)grown in Binzhou,Shandong

为了解山东滨州冬枣产区土壤中丛枝菌根(AM)真菌资源种类、性状,从沾化区5处冬枣园采集枣树根围土样,分离丛枝菌根真菌。采用湿筛倾注-蔗糖离心法分析真菌的群落组成,共分离到丛枝菌根真菌5属26种,其中球囊霉属10种、近明囊霉属2种、斗管囊霉属3种、根孢囊霉属4种、无梗囊霉属7种。5处采样园中,金平枣园的AM真菌种类22种,大高东石村枣园21种,下洼金平枣园16种。测定其物种丰度、孢子密度、多样性等参数,结果表明,变形球囊霉在各采样园均有发现,其中大高东石村园、下洼金平园、平家村园的频度均为100%,下洼东平园、泊头镇园均为66.6%;白色球囊霉、近明球囊霉、摩西斗管囊霉、疣突斗管囊霉、根内根孢囊霉、木薯根孢囊霉、光壁无梗囊霉、双网无梗囊霉在各采样园出现频度也较高。

In order to understand the distribution of Arbuscular mycorrhizal(AM)fungi in the soil of Dongzao jujube orchards in Binzhou,the root soil samples were collected from 6 jujube orchards in Zhanhua in September 2020.AM fungi were isolated and species abundance,spore density,and diversity index of AM fungi were measured.A total of 26 species of AM fungi belonging to 5 genera,including 10 species of Claroideoglomus,2 species of Glomus,3 species of Funneliformis,4 species of Rhizophagus,and 7 species of Acaulospora,were isolated by centrifugation with a wet sieve pouring sucrose.Among all the sampling sites,Jinping Zaoyuan(No.3)had the most AM fungi species(22 species),followed by Dagaodongshi Village jujube garden(21 species),and Xiawajinping(16 species).The frequency of G.versiforme in Dagaodongshi Village,Xiawajinping,and Hepingjia was 100%.In Xiawadongping and Botou Town,it was 66.6%,and it was found in all sampling sites.All samples contained G.albidum,C.claroideum,F.mosseae,F.verruculosum,R.intraradices,R.manihotis,A.laevis and A.bireticulata.To investigate the diversity and distribution of AM fungi in different jujube orchards,and to provide a basis for the development and application of AM fungi germplasm resources in Dongzao jujube orchards in Binzhou at the same time,it is of practical significance to collect,screen and preserve AM fungi in the future,and to develop special AM fungi for jujube trees.AM fungi can improve the yield,stress resistance and fruit quality of Dongzao jujube,which provides the basic data for further research.