外源甜菜碱对盐胁迫杜梨种子的萌发及生理特性研究

Effects of exogenous betaine on the seeds germination and physiological characteristics of Pyrus betulaefolia seedlings

探讨5个浓度0、0.5、1.0、5.0、10.0 mmol/L的外源甜菜碱对100 mmol/LNaCl胁迫下杜梨种子萌发、幼苗生长及其生理特性的影响,以不添加甜菜碱的处理作为对照。结果表明,甜菜碱处理对盐胁迫下杜梨种子发芽、幼苗生长及生理代谢均有显著促进作用。其中以1.0 mmol/L浓度处理的效果最显著,杜梨种子的萌发率、发芽势、幼苗株高、地上部鲜重、叶片超氧化物歧化酶、过氧化物酶和过氧化氢酶活性、叶绿素含量、净光合速率均显著高于对照和另外3个浓度处理;丙二醛含量均低于对照和另3个处理。

The effects of five concentrations of exogenous betaine(0,0.5,1.0,5.0,10.0 mmol/L)on the seeds germination,seedling growth,and physiological characteristics of Pyrus betulaefolia under 100 mmol/L NaCl were investigated,with the treatment without betaine as the control.The results indicated that treatments with exogenous betaine at various concentrations significantly promoted the germination of P.betulaefolia seeds and the aboveground growth of seedlings under salt stress,with the treatment of 1.0 mmol/L betaine showing the most significant impact.The germination rate and germination potential,seedling height,aboveground fresh weight,leaf superoxide dismutase,peroxidase and catalase activities,chlorophyll content,and net photosynthetic rate were significantly higher than those of the control and the other three treatments;while the MDA content was lower than that of the control and the other three treatments.