基于TCGA和GEO数据库分析MAFG在肝癌中的表达及临床意义

Expression of MAFG in hepatocellular carcinoma and its clinical significance via TCGA and GEO databases

目的通过对TCGA和GEO数据库中肝细胞肝癌(hepatocellular carcinoma,HCC)的数据挖掘,探讨V-maf鸟类肌筋膜纤维肉瘤癌基因同源物G(V-maf avian musculoaoneurotic fibroscarcoma oncogene homolog G,MAFG)基因在HCC中的表达及临床意义。方法通过TCGA和GEO数据库分析MAFG在HCC组织及癌旁组织的表达差异,通过HPA数据库分析MAFG表达水平与患者预后的关系。采用实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)法检测HCC组织中MAFG的表达水平。利用TCGA R软件,对交集差异基因进行基因本体论(gene ontology,GO)功能注释和京都基因与基因百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果TCGA和GEO数据集分析均发现MAFG在HCC组织中呈显著高表达(P<0.05)。RT-qPCR验证结果显示,与癌旁组织相比,MAFG在HCC组织中表达显著增高(P<0.05)。MAFG高表达组HCC患者5年内的生存率要低于MAFG低表达的患者。MAFG可能通过细胞分化、细胞迁移、炎性反应等生物学过程以及代谢通路、P53信号通路等信号通路进一步影响HCC发生、发展及预后。结论MAFG在HCC组织中呈高表达,其高表达预示HCC患者预后不佳,可为日后HCC的诊断和治疗提供新思路。

Objective To evaluate the clinical significance of MAFG expression in hepatocellular carcinoma(HCC)samples through data mining from TCGA and GEO database.Methods The differences in MAFG gene expression and clinicopathological features between tumor and adjacent tissues were analyzed via GEO and TCGA database,and the relationship between MAFG expression levels and patient prognosis was analyzed through the Human Protein Atlas(HPA)database.Meanwhile,the RT-qPCR method was used to detect the expression of MAFG in HCC tissues.The TCGA R software was employed to conduct gene ontology(GO)functional annotation and kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.Results MAFG expression was significantly higher(P<0.05)in HCC samples in TCGA and GEO database.Results verified by RT-qPCR showed that MAFG expression was higher(P<0.05)in HCC tissues than in adjacent tissues.Survival analysis further showed that the survival time of HCC patients with high expression of MAFG was significantly shorter than that of patients with low expression.It is indicated that MAFG may further influence the occurrence,development,and prognosis by biological processes such as cell division,cell migration,inflammatory response,protein homodimerization activity,and signaling pathways such as metabolic pathway,drug metabolism-cytochrome P450,P53 signaling pathway,and chemical carcinogenesis.Conclusion MAFG was highly expressed in HCC tissues,indicating poor prognosis for HCC patients,which provides new ideas for the diagnosis and treatment of HCC in the future.