血清miR-25、miR-873水平与冠心病患者心功能及冠状动脉狭窄程度的相关性

Correlation between serum levels of miR-25 and miR-873 and cardiac function and degree of coronary artery stenosis in patients with coronary heart disease

目的探讨血清微小RNA-25(miR-25)、微小RNA-873(miR-873)水平与冠心病(CHD)患者心功能及冠状动脉狭窄程度的相关性。方法选取2019年8月—2020年10月本院收治的100例经冠状动脉造影的检查确诊的CHD患者为研究对象(CHD组),另选取同期在我院进行体检的100例健康体检者为对照组,比较两组血清miR-25、miR-873水平。根据美国纽约心脏病协会(NYHA)心功能分级,将CHD患者分为NYHAⅠ~Ⅱ级38例、Ⅲ级35例、Ⅳ级27例,比较各组血清miR-25、miR-873水平。根据Gensini评分,将所有CHD患者分为轻度组(35例)、中度组(41例)和重度组(24例),比较各组血清miR-25、miR-873水平。Pearson法分析CHD患者血清miR-25、miR-873水平与心功能指标[左室射血分数(LVEF)、左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)]及冠状动脉狭窄程度的关系。结果CHD组患者血清miR-25、miR-873水平低于对照组(P<0.05)。NYHAⅢ级和NYHAⅣ级患者血清miR-25、miR-873水平低于NYHAⅠ~Ⅱ级患者(P<0.05);NYHAⅣ级患者血清miR-25、miR-873水平低于NYHAⅢ级患者(P<0.05)。随着轻、中、重度病情程度的增加,血清miR-25、miR-873水平及LVEF依次降低,LVEDD、LVESD水平及Gensini评分依次升高(P<0.05)。相关性分析显示,Gensini评分、LVEDD、LVESD与血清miR-25、miR-873水平呈负相关(P<0.05);LVEF与血清miR-25、miR-873水平呈正相关(P<0.05)。结论CHD患者血清miR-25、miR-873水平降低,且二者与心功能及冠状动脉狭窄程度显著相关。

Objective To investigate the correlation between serum levels of microRNA-25(miR-25)and microRNA-873(miR-873)with cardiac function and degree of coronary artery stenosis in patients with coronary heart disease(CHD).Methods One-hundred CHD patients diagnosed by coronary angiography admitted to our hospital from August 2019 to October 2020 were collected as the study subjects(CHD group),100 healthy individuals who were examined in our hospital during the same period were collected as the control group,and the serum levels of miR-25 and miR-873 were compared between the two groups.According to the NYHA cardiac function grading,there were 38 CHD patients of NYHA gradeⅠ-Ⅱ,35 of NYHA gradeⅢ,and 27 of NYHA gradeⅣ.The serum levels of miR-25 and miR-873 were compared among each group.According to the Gensini score,all CHD patients were grouped into mild group(35 cases),moderate group(41 cases),and severe group(24 cases),and the serum levels of miR-25 and miR-873 were compared in each group.Pearson method was applied to analyze the relationship between serum miR-25 and miR-873 levels,cardiac function indicators,and the degree of coronary artery stenosis in CHD patients.Results The serum levels of miR-25 and miR-873 in the CHD group were lower than those in the control group(P<0.05).The serum levels of miR-25 and miR-873 in NYHAⅢand NYHAⅣpatients were lower than those in NYHAⅠ-Ⅱpatients(P<0.05);the serum levels of miR-25 and miR-873 in NYHA gradeⅣpatients were lower than those in NYHA gradeⅢpatients(P<0.05).As the severity of mild,moderate,and severe illness increased,serum levels of miR-25,miR-873,and LVEF decreased sequentially,while LVEDD LVESD levels and Gensini score increased sequentially(P<0.05).Correlation analysis showed that Gensini score,LVEDD,and LVESD were negatively correlated with serum levels of miR-25 and miR-873(P<0.05);LVEF was positively correlated with serum levels of miR-25 and miR-873(P<0.05).Conclusion The serum levels of miR-25 and miR-873 in CHD patients decrease,and they are obviously correlated with cardiac function and the degree of coronary artery stenosis.