化学感受蛋白基因OasiCSP4在亚洲小车蝗型变过程中的表达谱分析

Expression profile analysis of chemosensory protein gene OasiCSP4 in Oedaleus asiaticus during phase transition

【目的】揭示化学感受蛋白基因OasiCSP4在亚洲小车蝗两型转变过程中的表达模式,为OasiCSP4功能研究奠定基础。【方法】将亚洲小车蝗雌、雄虫分别在群居型散居化处理和散居型群居化处理0、1、6、12、24、72 h后解剖为头、胸、腹、翅、触角、前足、中足、后足8个组织,采用实时荧光定量PCR技术测定雌、雄虫不同时间、不同组织中的OasiCSP4表达谱并对其表达趋势进行分析。【结果】在群居型散居化处理过程中,雄虫前足OasiCSP4相对表达水平显著高于其他组织(P<0.05);处理0、6、24、72 h雌虫前足OasiCSP4相对表达水平均显著高于其他组织(P<0.05),处理1 h雌虫头部OasiCSP4相对表达水平最高,处理12 h雌虫中足OasiCSP4相对表达水平显著高于其他组织(P<0.05);雄虫头、触角和中足OasiCSP4相对表达水平随处理时间延长而降低,处理6 h雄虫翅、前足和雌虫头、翅、触角、前足OasiCSP4相对表达水平最高。在散居型群居化处理过程中,OasiCSP4在雌虫前足的相对表达水平与其他组织相比差异显著(P<0.05);除处理0、1、72 h OasiCSP4在雄虫中足高表达外,其他处理时间下均在雄虫前足高表达;OasiCSP4在雄虫头、翅和雌、雄虫触角、前足中的相对表达水平随处理时间延长而降低,处理6 h雌虫头和中足OasiCSP4相对表达水最高。【结论】化学感受蛋白基因OasiCSP4在亚洲小车蝗两型转变过程中差异表达。

【Objective】To reveal the expression patterns of the chemosensory protein gene OasiCSP4 of Oedaleus asiaticus during phase transition,laying a foundation for the research of OasiCSP4 gene function.【Methods】The male and female O.asiaticus were dissected into eight tissues,including head,thorax,abdomen,wing,antennae,forelegs,midlegs and hindlegs in the 0,1,6,12,24 and 72 h of the isolation of gregarious and the crowding of solitary.qRT-PCR technology was used to determine the expression patterns and expression trend of OasiCSP4 in male and female O.asiaticus at different times and in different tissues.【Results】In the isolation of gregarious O.asiaticus,the relative expression level of OasiCSP4 of male forelegs was significantly higher than that of other tissues(P<0.05).Treatment for 0,6,24,72 h,the relative expression level of OasiCSP4 of female forelegs were also significantly higher than that of other tissues(P<0.05).Treatment for 1 h,the relative expression level of OasiCSP4 of female head was the highest.Treatment for 12 h,the relative expression level of OasiCSP4 in the female midlegs were also significantly higher than that of other tissues(P<0.05).The relative expression level of OasiCSP4 in male head,antennae and midlegs decreased with the prolongation of treatment time.Treatment for 6 h,the relative expression level of OasiCSP4 in male wing,forelegs and female head,wing,antennae and forelegs were the highest.During the crowding of solitary,the relative expression level of OasiCSP4 in the foreleg of female showed significant differences compared to other tissues(P<0.05).Except for the high expression of OasiCSP4 in male midlegs at 0,1,and 72 hours of treatment,it was highly expressed in male forelegs at all other treatment times.The relative expression levels of OasiCSP4 in male heads,wings,and female and male antennae,forelegs decreased with prolonged treatment time,with the highest relative expression levels observed in female heads and midlegs after 6 hours of treatment.【Conclusion】Chemosensory protein gene OasiCSP4 was differentially expressed during the phase transition in O.asiaticus.