寄主植物代谢物对沙葱萤叶甲取食的影响

Effects of host plant metabolites on feeding of Galeruca daurica

【目的】揭示寄主植物代谢物对沙葱萤叶甲取食量和取食偏好的影响。【方法】选取沙葱萤叶甲寄主植物的6种代谢物(3种次级代谢物,分别为异槲皮苷、异黄酮和芦丁;3种初级代谢物,分别为D-半乳糖、β-D-葡萄糖和L-鼠李糖),以10%DMSO溶液分别配制0.01 mmol/L(低浓度)、0.10 mmol/L(中浓度)和1.00 mmol/L(高浓度)6种代谢物处理液浸泡韭菜,饲喂沙葱萤叶甲,以饲喂10%DMSO溶液浸泡的韭菜为对照组,测定沙葱萤叶甲(幼虫、成虫)对不同寄主代谢物的取食量;应用二项叶碟选择法研究沙葱萤叶甲对不同代谢物及不同浓度处理组的取食偏好。【结果】沙葱萤叶甲幼虫对高浓度异槲皮苷处理的取食量显著低于对照组(P<0.05),对中、高浓度L-鼠李糖处理的取食量显著高于对照组(P<0.05);沙葱萤叶甲成虫对低、中浓度异黄酮和低、高浓度L-鼠李糖处理的取食量显著高于对照组(P<0.05)。沙葱萤叶甲幼虫对中、高浓度异黄酮,高浓度芦丁和低浓度β-D-葡萄糖处理的取食偏好显著低于对照组(P<0.05),而对低浓度L-鼠李糖处理的取食偏好极显著高于对照组(P<0.01);沙葱萤叶甲成虫对高浓度异槲皮苷处理的取食偏好极显著高于对照组(P<0.01),而对高浓度异黄酮、芦丁和D-半乳糖处理的取食偏好显著低于对照组(P<0.01)。【结论】寄主植物代谢物对沙葱萤叶甲取食的影响与代谢物种类、含量以及沙葱萤叶甲的发育阶段有关。

【Objective】To reveal the effects of host plant metabolites on the feeding intake and preference of Galeruca daurica.【Methods】6 metabolites of the host plant of G.daurica(including 3 secondary metabolites,namely isoquercitrin,isoflavone and rutin,respectively,and 3 primary metabolites,namely D-galactose,β-D-glucose and L-rhamnose)were selected,and soaked in 10%DMSO solution to prepare 6 metabolite treatment solutions of 0.01 mmol/L(low concentration),0.10 mmol/L(medium concentration),and 1.00 mmol/L(high concentration)in chives,to feed G.daurica.The treatment soaked in 10%DMSO solution in chives was used as the control group.The food intake of different host metabolites by G.daurica(larvae,adults)was measured.The binomial leaf dish selection method was used to study the feeding preference on different host plant metabolites and different concentration treatment groups.【Results】The food intake of larvae on the high concentration isoquercetin treatment was significantly less than control(P<0.05),whereas the food intakes of larvae on the middle and high concentration L-rhamnose treatments were significantly more than control(P<0.05).The food intakes of adults on the low and middle concentration isoflavone,and low and high concentration L-rhamnose treatments were significantly more than control(P<0.05).The feeding preference of larvae on the middle and high concentration isoflavone,high concentration rutin,and low concentrationβ-D-glucose treatments were significantly less than control(P<0.05),whereas the feeding preference of larvae on the low concentration L-rhamnose treatment was extremely significantly more than control(P<0.01).The feeding preference of adults on the high concentration isoquercetin treatment group was extremely significantly more than control(P<0.01),whereas the feeding preference indices of adults on the high concentration isoflavone,rutin and D-galactose treatments were much significantly less than control(P<0.01).【Conclusion】Effects of host plant metabolites on feeding by G.daurica depend on their kinds,contents,and developmental stages of G.daurica.