乌梅对脾虚泄泻模型大鼠的作用研究

Effect of MUME FRUCTUS on Rat Model of Pixuxiexie(脾虚泄泻)Syndrome

目的:探究乌梅对脾虚泄泻模型大鼠的止泻作用及其作用机制。方法:灌胃大黄水煎液复制脾虚泄泻大鼠模型,给予乌梅水煎液或蒙脱石散干预5 d,观察大鼠一般情况,评判大鼠布里斯托(Bristol)粪便性状评分、测定大鼠稀便率、大便含水率、小肠推进率、胃残留率;测定血清胃动素(MLT)、胃泌素(GAS)和结肠中Na+-K+-ATP酶含量;观察结肠病理切片;免疫荧光法检测结肠水通道蛋白3(AQP3)、AQP4的表达。结果:与正常对照组比较,模型对照组大鼠稀溏明显,Bristol粪便性状评分、稀便率和大便含水率均显著升高,体质量显著降低,小肠推进率显著升高,胃残留率显著降低,血清MTL含量明显升高,血清GAS含量显著降低,结肠Na+-K+-ATP酶含量显著降低(P<0.01),光镜下可见结肠组织明显的炎症浸润改变、黏膜缺损与腺体结构分离,结肠AQP3、AQP4蛋白相对表达量显著降低(P<0.01);与模型对照组比较,乌梅各剂量组大鼠粪便稀溏好转,Bristol粪便性状评分、稀便率和大便含水率显著降低,小肠推进率显著降低,胃残留率显著增加(P<0.01),血清MTL、GAS含量明显降低(P<0.05或P<0.01),结肠Na+-K+-ATP酶含量、结肠AQP4蛋白表达显著升高(P<0.01),光镜下可见结肠组织增生及黏膜损伤改善;乌梅水煎液10 g/kg组体质量明显升高(P<0.05),乌梅水煎液2.5、5 g/kg组结肠AQP3蛋白表达明显升高(P<0.05或P<0.01)。结论:乌梅对脾虚泄泻大鼠有显著止泻作用,其具体机制与降低MLT、GAS含量,抑制胃肠运动亢进;调节Na+-K+-ATP酶活性,增强AQP3、AQP4表达,降低大便含水率;改善粘膜损伤,改善炎症浸润表现等有关。

Objective:To investigate the anti-diarrheal effect and mechanism of MUME FRUCTUS on the Pixuxiexie syndrome(PXXX)model rats.Methods:The PXXX rat model was established by gavage with RHEI RADIX ET RHIZOMA decoction.MUME FRUCTUS decoction or montmorillonite powder was given for 5 days.The general conditions of the rats were observed,and Bristol stool form scale,fecal consistency,fecal water content,small intestinal propulsion rate,and gastric residue rate were measured.Serum motilin(MLT),gastrin(GAS),and colonic Na+-K+-ATPase content were measured.Colonic pathological sections were observed,and immunofluorescence was used to detect the expression of colonic aquaporin Protein-3(AQP3)and AQP4.Results:Compared with the normal control group,the model control group showed significant loose stools,with significantly increased Bristol stool form scale,fecal consistency,and fecal water content,while body weight significantly decreased,small intestinal propulsion rate significantly increased,and gastric residue rate significantly decreased(P<0.01).Serum MLT content increased significantly(P<0.05),serum GAS content decreased significantly(P<0.01),colonic Na+-K+-ATPase content decreased significantly(P<0.01),obvious inflammation infiltration changes,mucosal defects,and glandular structure separation were observed under light microscope,and the relative expression levels of colonic AQP3 and AQP4 proteins decreased significantly(P<0.01).Compared with the model control group,each dose group of MUME FRUCTUS showed improvement diarrhea in PXXX model,with significantly decreased Bristol stool form scale,fecal consistency,and fecal water content(P<0.01),significantly decreased small intestinal propulsion rate,significantly increased gastric residue rate(P<0.01),significantly decreased serum MLT and GAS content(P<0.05 or P<0.01),significantly increased colonic Na+-K+-ATPase content,significantly increased colonic AQP4 protein expression(P<0.01),proliferation of colonic tissue and improvement of mucosal damage were observed under light microscope.The body weight of rats in the MUME FRUCTUS decoction 10 g/kg group increased significantly(P<0.05),and the expression of colonic AQP3 in the MUME FRUCTUS decoction 2.5 g/kg and 5 g/kg groups increased significantly(P<0.05 or P<0.01).Conclusion:MUME FRUCTUS has a significant anti-diarrheal effect on PXXX rats.Its specific mechanism is related to the reduction of MLT,GAS content,inhibition of gastrointestinal hyperactivity,regulation of Na+-K+-ATPase activity,enhancement of AQP3,AQP4 expression,and reduction of fecal water content.It also improves mucosal damage,inflammation infiltration,and other manifestations.