胃癌组织中miR-155-5p、miR-4286表达与胃癌合并Hp感染患者临床特征及其预后的关系

The relationship between miR-155-5p and miR-4286 expression in gastric cancer tissue and clinical features and prognosis of patients with gastric cancer complicated with Hp infection

目的探究胃癌组织中微小RNA(miR)-155-5p、miR-4286表达与胃癌合并幽门螺旋杆菌(Hp)感染患者临床特征及其预后的关系。方法选取2020年1月-2022年6月于本院接受手术切除治疗的144例胃癌患者,根据是否感染Hp分为Hp阳性组(n=93)和Hp阴性组(n=51)。随访期间若胃癌合并Hp感染患者癌症复发或全因死亡归为预后不良(n=34),否则归为预后良好(n=59)。采用实时荧光定量PCR法(qRT-PCR)测定癌组织、癌旁组织miR-155-5p、miR-4286的表达水平。logistic回归分析胃癌合并Hp感染患者预后不良的影响因素。绘制受试者工作特征曲线(ROC)分析癌组织miR-155-5p、miR-4286对胃癌合并Hp感染患者预后不良的预测价值。结果与癌旁组织相比,胃癌患者癌组织miR-155-5p、miR-4286表达均上升(P<0.05)。与Hp阴性组相比,Hp阳性组miR-155-5p、miR-4286表达均上升(P<0.05)。癌组织miR-155-5p、miR-4286表达与胃癌合并Hp感染患者的肿瘤直径、TNM分期、淋巴结转移有关(P<0.05)。与预后良好组相比,预后不良组miR-155-5p、miR-4286表达均上升(P<0.05)。miR-155-5p、miR-4286为胃癌合并Hp感染患者预后的危险因素(P<0.05)。癌组织miR-155-5p、miR-4268联合预测胃癌合并Hp感染患者预后不良的曲线下面积(AUC)为0.978,显著大于miR-155-5p(Z=2.236,P=0.025)、miR-4268(Z=2.648,P=0.008)单独预测的AUC。结论胃癌合并Hp感染患者癌组织miR-155-5p、miR-4286均呈高表达,与患者的临床特征及预后不良有关,二者联合具有较高的胃癌合并Hp感染患者预后不良评估价值。

Objective To investigate the relationship between the expression of microRNA(miR-155-5p)and miR-4286 in gastric cancer tissue and the clinical features and prognosis of patients with gastric cancer complicated with Helicobacterpylori(Hp)infection.NMethods From January 2020 to June 2022,144 gastric cancer patients who underwent surgical resection treatment in our hospital were selected.According to whether they were infected with Hp,they were assigned into Hp positive group(n=93)and Hp negative group(n=51).Patients with gastric cancer complicated with Hp infection who experienced cancer recurrence or all-cause death during follow-up were classified as poor prognosis(n=34),otherwise they were classified as good prognosis(n=59).Real-time fluorescence quantitative PCR(qRT-PCR)was applied to measure the expression levels of miR-155-5p and miR-4286 in cancer tissues and adjacent tissues.Logistic regression was applied to analyze the influencing factors of poor prognosis in gastric cancer patients with Hp infection.Receiver operating characteristic(ROC)curves were plotted to analyze the predictive value of miR-155-5p and miR-4286 in cancer tissue for poor prognosis in gastric cancer patients with Hp infection.ResultsCompared with adjacent tissues,the expression of miR-155-5p and miR-4286 in cancer tissues of gastric cancer patients increased(P<0.05).Compared with the Hp negative group,the expression of miR-155-5p and miR-4286 increased in the Hp positive group(P<0.05).The expression of miR-155-5p and miR-4286 in cancer tissues was associated with tumor diameter,TNM staging,and lymph node metastasis in gastric cancer patients with Hp infection(P<0.05).Compared with the good prognosis group,the expression of miR-155-5p and miR-4286 increased in the poor prognosis group(P<0.05).MiR-155-5p and miR-4286 were risk factors for the prognosis of gastric cancer patients with Hp infection(P<0.05).The area under the curve(AUC)of miR-155-5p combined with miR-4268 in predicting poor prognosis in gastric cancer patients with Hp infection was 0.978,obviously higher than the AUC predicted by miR-155-5p(Z=2.236,P=0.025)andmiR-4268(Z=2.648,P=0.008)alone.C.onclusionMiR-155-5p and miR-4286 are highly expressed in cancer tissues of patients with gastric cancer complicated with Hp infection,which is related to the clinical features and poor prognosis of the patients.The combination of the two has a high value in evaluating the poor prognosis of patients with gastric cancer complicated with Hp infection.