过氧甲酸预处理与紫外联合消毒真菌效能机制

Efficacy and mechanism of combined inactivation of fungal spores by peracetic acid pretreatment and UV combined

本文探讨预暴露过氧甲酸(PFA)与紫外光(UV)联合PFA处理对真菌孢子灭活效能及机制的影响.研究发现,PFA作为一种有效的消毒剂,在低压紫外光激活下能够产生强氧化作用,破坏真菌孢子的细胞壁结构,进而达到灭活目的.结果表明,PFA的预处理能够加强后续的UV/PFA联合消毒过程,在相同的UV和PFA剂量条件下,经过12min的PFA预处理后再联合UV灭活,黑曲霉和黄曲霉的灭活率分别提升至3.67-log和2.51-log,是直接UV/PFA处理效果的1.21和1.17倍.此外,研究还考察预处理时间、PFA浓度、p H值以及腐殖酸浓度等因素对灭活效果的影响.增大预处理时间和PFA浓度能显著提高灭活效率,而碱性条件下和高腐殖酸浓度不利于灭活效果.流式细胞仪分析揭示PFA-UV/PFA联合处理对细胞膜的破坏更为严重,且活性氧水平显著提高.更多的羟基自由基生成量及更强的协同作用是灭活效果提升的关键因素.此外,PFA-UV/PFA处理对于未彻底灭活的真菌孢子的再生能力抑制效果优于单纯的UV/PFA处理.相较于PAA-UV/PAA体系,PFA-UV/PFA联合灭活体系在灭活真菌孢子方面展现出更高的潜力和效率.

The study investigated the effects and mechanisms of fungal spore inactivation by pre-exposure to peracetic acid(PFA)combined with ultraviolet(UV)light and subsequent PFA treatment.It was found that PFA,as an effective disinfectant,could produce strong oxidative effects under low-pressure UV activation,disrupting the cell wall structure of fungal spores and thereby achieving inactivation.The results indicated that PFA pretreatment could enhance the subsequent UV/PFA combined disinfection process.Under the same UV and PFA dosage conditions,after 12minutes of PFA pretreatment followed by UV inactivation,the inactivation rates of Aspergillus niger and Aspergillus flavus increased to 3.67-log and 2.51-log,respectively,which were 1.21 and 1.17 times the effect of direct UV/PFA treatment.Additionally,the study examined the influence of pretreatment time,PFA concentration,pH value,and humic acid concentration on the inactivation effect.Increasing the pretreatment time and PFA concentration significantly improved the inactivation efficiency,while alkaline conditions and high humic acid concentrations were detrimental to the inactivation effect.Flow cytometry analysis revealed that the PFA-UV/PFA combined treatment caused more severe damage to the cell membrane and significantly increased reactive oxygen species levels.The generation of more hydroxyl radicals and stronger synergistic effects were key factors in enhancing the inactivation effect.Furthermore,the PFA-UV/PFA treatment was more effective in inhibiting the regeneration ability of incompletely inactivated fungal spores compared to the UV/PFA treatment alone.Compared to the PAA-UV/PAA system,the PFA-UV/PFA combined inactivation system demonstrated higher potential and efficiency in inactivating fungal spores.