miRNA-495-3p、Hillday交叉识别蛋白在喉癌组织中的表达及其临床意义

Expressions of miRNA-495-3P and Hillday cross-recognition protein in laryngeal carcinoma and their clinical significances

目的探讨喉癌组织中miRNA-495-3p(miR-495-3p)、Hillday交叉识别蛋白(HJURP)的表达和相关性,及其与患者临床病理特征的关系。方法选择2023年6月至10月郑州大学第一附属医院30例原发性喉鳞状细胞癌患者的癌组织及相应的癌旁组织(距离癌组织边界1 cm以上)。采用实时荧光定量聚合酶链反应检测miR-495-3p相对表达量;蛋白质印迹法检测HJURP蛋白的相对表达量。采用Pearson法分析miR-495-3p和HJURP在喉癌组织中的相关性。结果与癌旁组织比较,miR-495-3p在喉癌组织中的相对表达量低(0.28±0.06比1.00±0.00),差异有统计学意义(t=-66.57,P<0.001);喉癌组织中HJURP蛋白的相对表达量高(10.2±5.6比1.0±0.0),差异有统计学意义(t=9.02,P<0.001)。Pearson相关分析结果显示,喉癌组织中miR-495-3p与HJURP呈负相关(r=-0.63,P<0.001)。有淋巴结转移患者miR-495-3p相对表达量低于无淋巴结转移患者(0.23±0.03比0.30±0.05),差异有统计学意义(t=-4.89,P<0.001);HJURP蛋白相对表达量高于无淋巴结转移患者(13.6±5.1比8.8±5.3),差异有统计学意义(t=2.33,P=0.030)。临床分期Ⅲ~Ⅳ期患者miR-495-3p相对表达量低于Ⅰ~Ⅱ期患者(0.23±0.03比0.32±0.04),差异有统计学意义(t=7.22,P<0.001);HJURP蛋白相对表达量高于Ⅰ~Ⅱ期患者(15.3±5.4比6.9±2.2),差异有统计学意义(t=-5.85,P<0.001)。结论喉癌中miR-495-3p表达降低,HJURP蛋白表达升高;二者具有相关性。

ObjectiveTo investigate the expressions and correlations of miRNA-495-3p(miR-495-3p)and Hillday cross-recognition protein(HJURP)in laryngeal carcinoma tissues,and their relationship with clinicopathological features of patients.MethodsCancerous tissues and corresponding paracancerous tissues(more than 1 cm away from the boundary of cancerous tissue)were selected from 30 patients with primary laryngeal squamous cell carcinoma in the First Affiliated Hospital of Zhengzhou University from June to October 2023.Real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression of miR-495-3p.The relative expression of HJURP protein was detected by Western blotting.The correlation between miR-495-3p and HJURP in laryngeal carcinoma was analyzed by Pearson method.ResultsCompared with paracancerous tissues,the relative expression of miR-495-3p in laryngeal cancer tissues was lower(0.28±0.06 vs.1.00±0.00),and the difference was statistically significant(t=-66.57,P<0.001);the relative expression of HJURP protein in laryngeal carcinoma tissues was higher(10.2±5.6 vs.1.0±0.0),and the difference was statistically significant(t=9.02,P<0.001).The results of Pearson correlation analysis showed that miR-495-3p was negatively correlated with HJURP in laryngeal cancer tissues(r=-0.63,P<0.001).The relative expression of miR-495-3p in patients with lymph node metastasis was lower than that in patients without lymph node metastasis(0.23±0.03 vs.0.30±0.05),and the difference was statistically significant(t=-4.89,P<0.001);the relative expression of HJURP protein was higher than that in patients without lymph node metastasis(13.6±5.1 vs.8.8±5.3),and the difference was statistically significant(t=2.33,P=0.030).The relative expression of miR-495-3p in patients with clinical stageⅢ-Ⅳwas lower than that in patients with stageⅠ-Ⅱ(0.23±0.03 vs.0.32±0.04),and the difference was statistically significant(t=7.22,P<0.001);the relative expression of HJURP protein was higher than that in patients with stageⅠ-Ⅱ(15.3±5.4 vs.6.9±2.2),and the difference was statistically significant(t=-5.85,P<0.001).ConclusionsIn laryngeal carcinoma,the expression of miR-495-3p is decreased,and the expression of HJURP protein is increased;the two are related.