青黛紧致健美霜对肥胖模型大鼠的减肥效果研究

Effects of Qingdai Jinzhi Jianmei Cream(青黛紧致健美霜)on weight reducing in obesity model rats

目的观察青黛紧致健美霜对大鼠肥胖尤其是腹部肥胖的预防效果,评价其减肥瘦身功效。方法将42只SPF级雄性SD大鼠采用随机数字表法分为正常对照组,模型对照组,空白基质组,青黛紧致健美霜低、中、高剂量组6组,每组7只。正常对照组给予普通维持饲料喂养,其余各组给予日粮型高脂饲料喂养诱导肥胖。造模同时,药物组大鼠于腹部脱毛处皮肤表面均匀涂抹青黛紧致健美霜,涂抹后按摩1 min,持续给药9周。通过观察大鼠体质量、颈腰围、Lee’s指数、脂体比、血脂水平、肝脏系数和组织病理学变化,探究青黛紧致健美霜的减肥效果。结果与正常对照组比较,模型对照组大鼠体质量变化率明显升高,差异有统计学意义(P<0.05);药物组大鼠的体质量增长较慢,中、高剂量组大鼠体质量变化率明显低于模型对照组(P<0.05)。与正常对照组比较,模型对照组大鼠腰围明显增大,Lee’s指数、肝脏系数及脂体比明显升高,差异均有统计学意义(P<0.05);与模型对照组比较,高剂量组大鼠腰围明显缩小,Lee’s指数和脂体比明显降低,差异均有统计学意义(P<0.05)。与正常对照组比较,模型对照组大鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平明显升高,高密度脂蛋白胆固醇(HDL-C)水平明显降低,差异均有统计学意义(P<0.05);与模型对照组比较,高剂量组大鼠血清LDL-C水平明显降低,差异有统计学意义(P<0.05)。肝脏和腹部皮下脂肪组织病理切片镜下观察显示,模型对照组大鼠的肝脏发生脂肪变性,腹部皮下脂肪分布密集,脂肪细胞变大;青黛紧致健美霜可明显降低肥胖模型大鼠的肝脏脂肪变性程度,减小腹部皮下脂肪细胞大小。结论青黛紧致健美霜对大鼠肥胖尤其是腹部肥胖具有显著的改善作用,减肥效果明显,且具有一定的降血脂作用。

Objective To observe the preventive impact of Qingdai Jinzhi Jianmei Cream(青黛紧致健美霜)on obesity in rats,particularly abdominal obesity,and to assess its efficacy in weight reducing.Methods 42 SPF male SD rats were divided into six groups(seven in each group)by the random number table method:normal control group,model control group,blank matrix group,Qingdai Jinzhi Jianmei Cream low-dose,medium-dose,and high-dose groups.The normal control group was provided with regular maintenance feed,while the other groups were fed high-fat feed to induce obesity.Simultaneously,the rats in the drug groups had Qingdai Jinzhi Jianmei Cream evenly applied to the skin surface of the abdominal hair removal area,followed by a one-minute massage after application,and the administration continued for nine weeks.By observing the alterations in body mass,neck and waist circumference,Lee's index,lipid-body ratio,blood fat level,liver coefficient,and histopathology of the rats,the effect of Qingdai Jinzhi Jianmei Cream on weight reducing was investigated.Results Compared with the normal control group,the change rate of body mass in the model control group was significantly elevated,and the difference was statistically significant(P<0.05).The body mass of rats in the drug groups increased at a slower pace,and the change rate of body mass in the medium and high-dose groups was significantly lower than that in the model control group(P<0.05).Compared with the normal control group,the waist circumference of rats in the model control group was significantly increased,and Lee's index,liver coefficient,and lipid-body ratio were significantly elevated,and the differences were statistically significant(P<0.05).Compared with the model control group,the waist circumference of rats in the high-dose group was significantly reduced,and Lee's index and lipid-body ratio were significantly decreased,which was statistically significant(P<0.05).Compared with the normal control group,the levels of TG,TC,and LDL-C in the model control group were significantly increased,while the level of HDL-C was significantly decreased,and the differences were statistically significant(P<0.05).Compared with the model control group,the level of LDL-C in the high-dose group was significantly reduced,which was statistically significant(P<0.05).Pathological observations of the liver and abdominal subcutaneous adipose tissue revealed that steatosis occurred in the liver of rats in the model control group,with a dense distribution of abdominal subcutaneous fat and enlarged fat cells.Qingdai Jinzhi Jianmei Cream can significantly reduce the degree of liver steatosis and the size of abdominal subcuta-neous fat cells in obese rats.Conclusion Qingdai Jinzhi Jianmei Cream can ameliorate the obesity of rats,especially abdominal obesity.Its effect on weight reducing is notable,and it has a certain effect of lowering blood fat.