基于生物打印技术构建的化妆品功效评价三维模型应用研究

Application of the 3D Models for Cosmetic Efficacy Evaluation Based on Bioprinting

采用生物打印技术,以甲基丙烯酰化明胶(gelatin methacryloyl,GelMA)、胶原混合胶作为生物墨水,模拟皮肤组织细胞外基质(extracellular matrix,ECM),分别构建了精度高且活力好的人永生化角质形成细胞Ha-CaT和小鼠黑素瘤细胞B16的三维细胞模型;随后,采用构建的三维细胞模型,对两种化妆品样品进行了保湿功效和美白功效评价研究。结果显示,样品组三维HaCaT细胞模型中水通道蛋白基因AQP3的表达量相比于空白对照组上调约1.08倍;三维B16细胞模型所检测样品的黑素抑制率约为20%。本研究构建的三维细胞模型结构立体,细胞存活良好,为化妆品功效检测行业探索了新的模型构建方式,且具有较好的市场应用前景。

Based on three-dimensional(3D)bioprinting,gelatin methacryloyl(GelMA)and collagen mixture were used as bioinks to simulate the extracellular matrix(ECM)of skin tissue,and standardized,high-precision,and dynamic 3D models of human immortalized epidermal cells(HaCaT)and mouse melanoma cells(B16)were constructed.The 3D cell models were used to evaluate the moisturizing and whitening effects of two cosmetic samples.The results showed that the expression level of AQP3 gene in the sample group detected by the 3D HaCaT model was up-regulated by 1.08 times compared with that in the negative control group,and the melanin inhibition rate reached about 20%in the sample group using the 3D B16 model.The constructed 3D cell models in this study proved to be with stable performance and good cell viability and may have great potential for application in efficacy testing in the cosmetics industry.